| 高、低糖甘蔗品种苗期糖分含量及代谢相关酶活性分析 |
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| 引用本文： | 牛俊奇,苗小荣,黄维,杨丽涛,李杨瑞.高、低糖甘蔗品种苗期糖分含量及代谢相关酶活性分析[J].热带作物学报,2019,40(4):681-687. |
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| 作者姓名： | 牛俊奇 苗小荣 黄维 杨丽涛 李杨瑞 |
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| 作者单位： | 1. 玉林师范学院生物与制药学院,广西玉林 5370002. 广西农产资源化学与生物技术重点实验室,广西玉林 5370003. 广西大学农学院,广西南宁 5300054. 中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所,广西南宁 530007 |
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| 基金项目： | 海南省重点研发计划-现代农业项目(ZDYF2018044);中国热带农业科学院环境与植物保护研究所自主选题项目(hzsjy2017005) |
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| 摘 要： | 以高糖(GT35)和低糖(B8)甘蔗品种苗期不同部位的叶和茎为材料,采用HPLC技术测定蔗糖、葡萄糖和果糖含量,分析2个甘蔗品种叶和茎中蔗糖代谢相关酶活性与糖分含量的相关性和差异性。结果表明:2个甘蔗品种苗期叶中蔗糖含量与SPS和SS-s酶活性呈显著正相关,茎中己糖含量与NI酶活性呈显著正相关,茎中蔗糖含量与SPS酶活性呈显著正相关,而与SS-c、SAI和CIN酶活性呈显著负相关。GT35茎中蔗糖含量显著高于B8,叶中蔗糖含量显著低于B8。GT35茎中SPS和叶中SS-s酶活性均显著高于B8,茎中SS-s和SS-c酶活性低于B8,其中只有幼茎中SS-c酶活性与B8差异不显著。说明苗期叶中高SS-s酶活性,茎中高SPS酶活性,低SS-s和SS-c酶活性,可能是调节高糖甘蔗品种苗期茎中蔗糖积累的重要因素。
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| 关 键 词： | 甘蔗 蔗糖代谢相关酶 糖分积累 相关性分析 |
| 收稿时间： | 2018-07-30 |
Relationship Between Sucrose Content and Sucrose Metabolism Enzymes in the High and Low Sugar Sugarcane at Seedling Stage |
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| NIU Junqi,MIAO Xiaorong,HUANG Wei,YANG Litao,LI Yangrui.Relationship Between Sucrose Content and Sucrose Metabolism Enzymes in the High and Low Sugar Sugarcane at Seedling Stage[J].Chinese Journal of Tropical Crops,2019,40(4):681-687. |
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| Authors: | NIU Junqi MIAO Xiaorong HUANG Wei YANG Litao LI Yangrui |
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| Institution: | 1. College of Biology and Pharmacy, Yulin Normal University, Yulin, Guangxi 537000, China2. Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Yulin, Guangxi 537000, China3. College of Agriculture, Guangxi University, Nanning, Guangxi 530005, China4. Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China |
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| Abstract: | The stems and leaves of different maturity at the seedling stage of high sugar (GT35) and low sugar (B8) sugarcane were used as the materials, and the contents of sucrose, glucose and fructose were measured using high performance liquid chromatography (HPLC), to analyze the correlation and difference between sucrose metabolic enzymes and sugar accumulation in high and low sugar sugarcane. The sucrose content in the leaves was positively correlated with the activity of sucrose phosphate synthase (SPS) and sucrose synthase in the synthesis direction (SS-s). The hexose content in the stem was positively correlated with the activity of neutral/alkaline invertase (NI), the sucrose content in the stem was positively correlated with the activity of SPS, and the activity of sucrose synthetase in the cleavage direction (SS-c), soluble acid invertase (SAI) and cell wall-bound invertase (CIN) was negatively correlated. Sucrose content in the stem of GT35 was significantly higher than that of B8, and sucrose content in the leaf was significantly lower than that of B8. The SPS activity in stems and SS-s activity leaves of GT35 were significantly higher than that of B8, while the SS-s and SS-c activity in GT35 stems was lower than that of B8, but only the SS-c activity in young stems of GT35 was not significantly lower than of B8. It indicates that high SS-s activity in leaves, high SPS activity in stems, low SS-s and SS-c activity in stems may be important factors for regulating sucrose accumulation in high-sugar varieties of sugarcane at seedling stage. |
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| Keywords: | sugarcane cultivars sucrose metabolism enzymes sucrose accumulation correlation analysis |
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