| 荔枝FKBP16-2基因启动子的克隆与瞬时表达分析 |
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| 引用本文： | 孙琴,孙进华,王树军,李焕苓,王家保.荔枝FKBP16-2基因启动子的克隆与瞬时表达分析[J].热带作物学报,2016,37(4):736-741. |
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| 作者姓名： | 孙琴 孙进华 王树军 李焕苓 王家保 |
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| 作者单位： | 1. 海南大学农学院,海南海口 570228;中国热带农业科学院环境与植物保护研究所,海南海口 571101;2. 中国热带农业科学院环境与植物保护研究所,海南海口,571101 |
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| 基金项目： | 国家现代农业产业技术体系(No.CARS-33-02);国家自然科学基金(No.31272137) |
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| 摘 要： | 前期研究中发现了一个果肉低表达而叶片中高表达的荔枝基因FKBP16-2。本文克隆了该基因1 578 bp的启动子片段并对其功能进行了初步分析,结果表明:荔枝FKBP16-2基因启动子序列中含有大量的TATA-box和CAAT-box保守元件,以及TCA-element,ARE,HSE,GCN4_motif,O2-site等各种转录调控相关的顺式作用元件。该启动子能驱动GUS基因在荔枝的花、叶、根、果皮以及种子中表达而在果肉中不表达,表达具有组织特异性。
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| 关 键 词： | 荔枝 FKBP16-2基因启动子 基因枪 顺式调控元件 瞬时表达 |
Cloning and Transient Expression Assay of FKBP16-2 Gene Promoter from Litchi |
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| SUN Qin,SUN Jinhu,WANG Shujun,LI Huanling and WANG Jiabao.Cloning and Transient Expression Assay of FKBP16-2 Gene Promoter from Litchi[J].Chinese Journal of Tropical Crops,2016,37(4):736-741. |
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| Authors: | SUN Qin SUN Jinhu WANG Shujun LI Huanling and WANG Jiabao |
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| Institution: | SUN Qin;SUN Jinhua;WANG Shujun;LI Huanling;WANG Jiabao;College of Agriculture, Hainan University;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences; |
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| Abstract: | A FKBP16-2 gene from litchi was found on previous studies, which expressed lower in the pulp and higher in the leaf. In this paper, a 1 578 bp promoter fragment of the gene was cloned and its functions were preliminarily analyzed. The results showed that the promoter sequence of FKBP16-2 gene from litchi contained a large number of TATA box and CAAT box, and several putative cis-elements, such as TCA-element, ARE, HSE, GCN4_motif, O2-site and so on. The promoter can drive GUS gene to express with tissue specificity in flower, leaf, root, pericarp and seed of litchi but not in pulp. |
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| Keywords: | Litchi FKBP16-2 gene promoter Particle bombardment Cis-regulatory element Transient expression |
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