Identification of Major Locus Bph35 Resistance to Brown Planthopper in Rice

An introgression line RBPH660,derived from wild rice Oryza rufipogon,showed stable resistance to brown planthopper(BPH).Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs.By using the bulked segregant analysis(BSA)-seq method,two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660,respectively.A major resistance locus,designated as Bph35 accounting for 51.27%of the phenotypic variation with a LOD score of 42.51,was mapped to the candidate region of chromosome 4 between In Del(insertion-deletion)markers PSM16 and R4 M13.For fine mapping of Bph35,one simple sequence repeat and three newly developed In Del markers were used to screen the recombinants.Finally,the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism(SNP)variant sites between the resistant and susceptible parents.Out of these genes,Os04 g0193950,encoding a putative NB-ARC(nucleotidebinding adaptor shared by APAF-1,R proteins and CED-4)and LRR(leucine-rich repeat)domain protein with nine non-synonymous SNP substitutions in its coding sequence regions,might be the candidate gene for Bph35.These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice.

Identification of Major Locus Bph35 Resistance to Brown Planthopper in Rice

An introgression line RBPH660, derived from wild rice Oryza rufipogon, showed stable resistance to brown planthopper(BPH). Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs. By using the bulked segregant analysis(BSA)-seq method, two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660, respectively. A major resistance locus, designated as Bph35 accounting for 51.27% of the phenotypic variation with a LOD score of 42.51, was mapped to the candidate region of chromosome 4 between In Del(insertion-deletion) markers PSM16 and R4 M13. For fine mapping of Bph35, one simple sequence repeat and three newly developed In Del markers were used to screen the recombinants. Finally, the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism(SNP) variant sites between the resistant and susceptible parents. Out of these genes, Os04 g0193950, encoding a putative NB-ARC(nucleotidebinding adaptor shared by APAF-1, R proteins and CED-4) and LRR(leucine-rich repeat) domain protein with nine non-synonymous SNP substitutions in its coding sequence regions, might be the candidate gene for Bph35. These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice.