| Calcium Signaling is Involved in Negative Phototropism ofRice Seminal Roots简 |
| |
| 引用本文： | CHEN Juan,岛 Yi-wei,XU Hua-wei.Calcium Signaling is Involved in Negative Phototropism ofRice Seminal Roots简[J].水稻科学,2014,21(1):39-46. |
| |
| 作者姓名： | CHEN Juan 岛 Yi-wei XU Hua-wei |
| |
| 摘 要： | Calcium ions(Ca2+) act as an intracellular second messenger and affect nearly all aspects of cellular life. They are functioned by interacting with polar auxin transport, and the negative phototropism of plant roots is caused by the transport of auxin from the irradiated side to the shaded side of the roots. To clarify the role of calcium signaling in the modulation of rice root negative phototropism, as well as the relationship between polar auxin transport and calcium signaling, calcium signaling reagents were used to treat rice seminal roots which were cultivated in hydroculture and unilaterally illuminated at an intensity of 100–200 μmol/(m2·s) for 24 h. Negative phototropism curvature and growth rate of rice roots were both promoted by exogenous CaCl2 lower than 100 μmol/L, but inhibited by calcium channel blockers(verapamil and LaCl3), calcineurin inhibitor(chlorpromazine, CPZ), and polar auxin transport inhibitor(N-1-naphthylphthalamic acid, NPA). Roots stopped growing and negative phototropism disappeared when the concentrations increased to 100 μmol/L verapamil, 12.500 μmol/L LaCl3, 60 μmol/L CPZ, and 6 μmol/L NPA. Moreover, 100 μmol/L CaCl2 could relieve the inhibition of LaCl3, verapamil and NPA. The enhanced negative phototropism curvature was caused by the transportation of more auxin from the irradiated side to the shaded side in the presence of exogenous Ca2+. Calcium signaling plays a key role as a second messenger in the process of light signal regulation of rice root growth and negative phototropism.
|
| 收稿时间： | 14 May 2013 |
Calcium Signaling is Involved in Negative Phototropism of Rice Seminal Roots |
| |
| Institution: | 1. Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of Chemistry and Life Science, Huanggang Normal University, Huanggang 438000, China;2. Institute of South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China;3. College of Life Science, Shaoxing University, Shaoxing 312000, China;4. College of Agriculture, Henan University of Science and Technology, Luoyang 471003, China;1. Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing 100097, PR China;2. School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu, PR China;1. National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310036, China;2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China;1. Department of Dermatology, Cochin Hospital, Assistance Publique des Hôpitaux de Paris and University of Paris Descartes, Paris, France;2. Division of Dermatology, Department of Medicine, University of Toronto, Toronto, Canada;3. Division of Dermatology, Women’s College Hospital, Toronto, Canada;1. Institute of Food and Crops Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, China;2. Life-Sciences School, Yunnan University, Kunming 650091, China |
| |
| Abstract: | Calcium ions (Ca2+) act as an intracellular second messenger and affect nearly all aspects of cellular life. They are functioned by interacting with polar auxin transport, and the negative phototropism of plant roots is caused by the transport of auxin from the irradiated side to the shaded side of the roots. To clarify the role of calcium signaling in the modulation of rice root negative phototropism, as well as the relationship between polar auxin transport and calcium signaling, calcium signaling reagents were used to treat rice seminal roots which were cultivated in hydroculture and unilaterally illuminated at an intensity of 100–200 μmol/(m2·s) for 24 h. Negative phototropism curvature and growth rate of rice roots were both promoted by exogenous CaCl2 lower than 100 μmol/L, but inhibited by calcium channel blockers (verapamil and LaCl3), calcineurin inhibitor (chlorpromazine, CPZ), and polar auxin transport inhibitor (N-1-naphthylphthalamic acid, NPA). Roots stopped growing and negative phototropism disappeared when the concentrations increased to 100 μmol/L verapamil, 12.500 μmol/L LaCl3, 60 μmol/L CPZ, and 6 μmol/L NPA. Moreover, 100 μmol/L CaCl2 could relieve the inhibition of LaCl3, verapamil and NPA. The enhanced negative phototropism curvature was caused by the transportation of more auxin from the irradiated side to the shaded side in the presence of exogenous Ca2+. Calcium signaling plays a key role as a second messenger in the process of light signal regulation of rice root growth and negative phototropism. |
| |
| Keywords: | calcium signaling polar auxin transport calcium channel blocker second messenger negative phototropism |
| 本文献已被 CNKI ScienceDirect 等数据库收录！ |
|
