Transferring Translucent Endosperm Mutant Gene Wx-mq and Rice Stripe Disease Resistance Gene Stv-b by Marker-Assisted Selection in Rice (Oryza sativa) |
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Authors: | Shu YAO Tao CHEN Ya-dong ZHANG Zhen ZHU Ling ZHAO Qing-yong ZHAO Li-hui ZHOU Cai-lin WANG |
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Institution: | aInstitute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R & D Center, Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China;bCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China |
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Abstract: | A high-yielding japonica rice variety, Wuyunjing 7, bred in Jiangsu Province, China as a female parent was crossed with a Japanese rice variety Kantou 194, which carries a rice stripe disease resistance gene Stv-bi and a translucent endosperm mutant gene Wx-mq. From F2 generations, a sequence characterized amplified region (SCAR) marker tightly linked with Stv-bi and a cleaved amplified polymorphic sequence (CAPS) marker for Wx-mq were used for marker-assisted selection. Finally, a new japonica rice line, Ning 9108, with excellent agronomic traits was obtained by multi-generational selection on stripe disease resistance and endosperm appearance. The utilization of the markers from genes related to rice quality and disease resistance was helpful not only for establishing a marker-assisted selection system of high-quality and disease resistance for rice but also for providing important intermediate materials and rapid selection method for good quality, disease resistance and high yield in rice breeding. |
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Keywords: | rice translucent endosperm mutant gene rice stripe disease resistance gene marker-assisted selection |
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