Marker-Assisted Selection of Xa21 Conferring Resistance to Bacterial Leaf Blight in indica Rice Cultivar LT2 |
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| Authors: | Hue Thi Nguyen Quang Hong Vu Tan Van Mai Thu Thi Nguyen Lam Duc Vu Tung Thanh Nguyen Long Viet Nguyen Hien Thu Thi Vu Hue Thi Nong Trung Nguyen Dinh Nakano Toshitsugu Liet Van Vu |
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| Institution: | 1. Crop Research and Development Institute, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi 10000, Vietnam;2. Faculty of Agronomy, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi 10000, Vietnam;3. Faculty of Biotechnology, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi 10000, Vietnam;4. Faculty of Land Management, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi 10000, Vietnam;5. Japan International Cooperation Agency, 16 Phan Chu Trinh, Hoan Kiem, Hanoi 10000, Vietnam |
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| Abstract: | Bacterial leaf blight of rice (BLB), caused by Xanthomonas oryzae pv. oryzae, is one of the most destructive diseases in Asian rice fields. A high-quality rice variety, LT2, was used as the recipient parent. IRBB21, which carries the Xa21 gene, was used as the donor parent. The resistance gene Xa21 was introduced into LT2 by marker-assisted backcrossing. Three Xoo races were used to inoculate the improved lines following the clipping method. Eleven BC3F3 lines carrying Xa21 were obtained based on molecular markers and agronomic performance. The 11 lines were then inoculated with the three Xoo races. All the 11 improved lines showed better resistance to BLB than the recipient parent LT2. Based on the level of resistance to BLB and their agronomic performance, five lines (BC3F3 5.1.5.1, BC3F3 5.1.5.12, BC3F3 8.5.6.44, BC3F3 9.5.4.1 and BC3F3 9.5.4.23) were selected as the most promising for commercial release. These improved lines could contribute to rice production in terms of food security. |
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| Keywords: | rice backcrossing bacterial leaf blight marker-assisted selection |
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