苦参毛状根的诱导培养及氧化苦参碱含量的测定

本文主要利用三种发根农杆菌A4、15834、R1601诱导苦参产生毛状根,初步为建立苦参的毛状根培养体系提供参考。本实验采用共培养法,考察了不同外植体、菌种、培养时间对苦参毛状根诱导率的影响,并对苦参毛状根中氧化苦参碱进行初步的含量测定。研究结果表明,苦参毛状根的最佳诱导条件是以生长1周的苦参幼芽为外植体,R1601为菌株,添加100μmol/l的乙酰丁香酮,侵染20 min,(25±1)℃暗处诱导,最终测定诱导率最高为27.6%,存活率为45.8%,而苦参毛状根中氧化苦参碱的富集量为1.68mg/g(苦参毛状根干重),未检测到苦参碱。

Induction of Hairy Roots and Determination of Oxymatrine in Sophora flavescens Ait.

this paper,hairy root of Sophora flavescens Ait.were induced by the infection of third Agrobacterium rhizogenes strains 15834,R1601 ,A4 and an in vitro culture system of the hairy roots was newly established.Hairy roots were induced by co-culture.Effects of explants, Agrobacterium rhizogenes,culture time on growth of hairy root were studied and Determination of Oxymatrine in Sophora flavescens Ait.The research shows that the highest induction frequency was obtained from young shoot with one week which were induced by R1601 for 10mins ,and increase of 100 p, mol/L acetosyringone. The transformation frequency was 27.6% ,annual survival rate was 45.8%. Hairy roots of Oxymatrine enrichment amount 1.68mg/g ,Total matrine content was not determined.