| 培养条件对平贝母愈伤组织生长和生物碱积累的影响研究 |
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| 引用本文： | 赵鸣,任越,李文龙,田义新.培养条件对平贝母愈伤组织生长和生物碱积累的影响研究[J].人参研究,2013(4):31-33. |
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| 作者姓名： | 赵鸣 任越 李文龙 田义新 |
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| 作者单位： | [1]吉林农业大学中药材学院,吉林长春130118 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]吉林敖东药业股份有限公司,吉林敦化133700 |
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| 基金项目： | 吉林省财政厅项目-平贝母品种选育研究和吉林省中医药管理局项目,项目编号：2010-pt072. |
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| 摘 要： | 目的 了解培养条件对平贝母愈伤组织生长和总生物碱含量的影响.方法 分别以光暗培养、氮源比例、碳源种类、蔗糖浓度、磷酸盐浓度作为影响因子进行愈伤组织培养.结果 光培养条件、NH4＋∶NO3-=1∶1、蔗糖4％、磷酸盐浓度1.5mmol· L-1时,平贝母愈伤组织生长较快、总生物碱积累较多,结论 培养条件对平贝母愈伤组织生长和总生物碱积累均有影响.
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| 关 键 词： | 培养条件 平贝母 愈伤组织 生物碱 |
Studies on the effects of culture conditions to growth and alkaloid accumulation of the callus from Fritillaria ussurionsis |
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| Authors: | Zhao Ming Ren Yue Li Wenlong Tian Yixin |
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| Institution: | Zhao Ming Ren Yue Li Wenlong Tian Yixin(1.College of TCM Materials, Jilin Agricultural University, Changchun 130118;2. Sc holl of Phtwmaceu- tical University, Shengyang 110016; 3. Jilin Aodong Yanbian Pharmaceutical Co. LTD., Dunhua City, 133700) |
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| Abstract: | Objective To understand the effects of cuhure conditions to growth and alkaloid accumulation of the callus from Fritillaria ussurionsis. Methods The conditions of light and dark culture, NH4＋：NO3- portion, different carbohydrates, sucrose concentration and phosphate were used as effective factors for the experiment. Results The condition of light, NH4＋：NO3- portion 1：1, sucrose concentration 4% and phosphate 1.5mmol ·L^-1 is optimal for the callus growth and alkaloid accumulation. Conclusion The culture conditions affect the growth and alkaloid accumulation of the callus from Fritillaria ussurionsis. |
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| Keywords: | Cuhure conditions Fritillaria ussurionsis Maxim Callus Alkaloid |
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