| 农杆菌介导法将抗草甘膦基因EPSPS转入玉米自交系的研究 |
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| 引用本文： | 孙传波,麻鹏达,陶蕊,郭嘉,李海华,孟凡梅,曲文利,李海华,刘文国,刘德璞,袁英.农杆菌介导法将抗草甘膦基因EPSPS转入玉米自交系的研究[J].玉米科学,2010,18(6):24-26. |
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| 作者姓名： | 孙传波 麻鹏达 陶蕊 郭嘉 李海华 孟凡梅 曲文利 李海华 刘文国 刘德璞 袁英 |
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| 作者单位： | 1. 吉林省农业科学院生物技术研究中心,长春,130033
2. 吉林省农业科学院玉米研究所,长春,130033 |
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| 基金项目： | 孙传波(1980-)，男，山东莒县人，硕士，从事玉米遗传转化研究。Tel：0431-87063719 |
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| 摘 要： | 通过农杆菌介导法将抗草甘膦基因EPSPS转入玉米自交系H99中,同时研究了农杆菌浓度、侵染时间及共生培养条件等因素对转化频率的影响。结果表明:菌液浓度OD600为0.6、侵染时间20 min、共生培养时间3 d为最佳转化条件。获得的转基因植株经PCR分析鉴定,其中7株表现阳性,证明外源基因已经整合到玉米基因组中。
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| 关 键 词： | 玉米 农杆菌介导 转化频率 EPSPS基因 |
| 收稿时间： | 2010/4/12 0:00:00 |
| 修稿时间： | 2010/5/19 0:00:00 |
Study on Agrobacterium tumefaciens Mediated Transformation of EPSPS Gene into Maize Inbred Line |
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| SUN Chuan-bo,MA Peng-d,TAO Rui,LIU Wen-guo,LIU De-pu,YUAN Ying,et al..Study on Agrobacterium tumefaciens Mediated Transformation of EPSPS Gene into Maize Inbred Line[J].Journal of Maize Sciences,2010,18(6):24-26. |
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| Authors: | SUN Chuan-bo MA Peng-d TAO Rui LIU Wen-guo LIU De-pu YUAN Ying |
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| Abstract: | The EPSPS gene was transferred into maize inbred line H99 by Agrobacterium tumefaciens mediated transformation. Factors influenced transformation efficiency was studied, including bacterial concentration, infection time and co-cultivation time. Results showed that bacterium concentration at OD600= 0.6, 20 minutes infection time and co-culture 3 days were the optimal conditions. PCR analysis on transformation plants demonstrated that 7 of them were positive and the foreign gene was integrated into maize genome. |
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