玉米幼苗叶表面蜡质含量及成分对干旱胁迫的响应

选用抗旱性不同的两个玉米自交系Mo17和B73为试验材料,采用盆栽控水试验,设置干旱和正常浇水两个处理,研究干旱胁迫对两个玉米品种3叶期幼苗叶面蜡质含量及成分变化的影响。结果表明,在干旱处理条件下,Mo17和B73的叶片失水率和叶绿素的抽提率均低于正常条件;两个品种叶面的蜡质含量均明显高于正常条件下的蜡质含量,且Mo17在正常和干旱条件下蜡质含量均高于B73。此外,在干旱条件下,蜡质合成相关的基因KCS、CER1、CER2、GL1、GL2和GL15的表达也明显增高。玉米叶片表面蜡质合成是应答干旱胁迫的一种重要方式,抗旱品种Mo17的抗旱性比B73要强,其表面蜡质合成较强是其中一个重要原因。

Changes of Wax Content in Leaves of Maize Seedlings under the Conditions of Drought Stress

Using two maize inbred line Mo17(drought resistant) and B73(drought sensitive) as the test materials to study the changes of leaf wax content and composition of maize seedlings under the conditions of drought(25%-35% of soil moisture) and normal water(70%-80% of soil moisture). The results showed that leaf water loss rate and extracted chlorophylls rate of Mo17 and B73 under drought condition were lower than that of Mo17 and B73 under normal condition. Leaf wax contents of Mo17 and B73 under drought condition were higher than that of Mo17 and B73 under normal condition. In addition, expression of wax synthesis related genes(KCS, CER1, CER2, GL1, GL2 and GL15) also increased under drought condition. The synthesis of maize leaf wax was an important way in response to drought stress. The drought resistance of Mo17 was stronger than that of B73, the main reason was that the ability of wax synthesis was stronger than that of B73.