| 抑制ZmCol3基因表达调控玉米开花期 |
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| 引用本文： | 贾伟,尹悦佳,柳青,刘洋,李楠,果天宇,郝东云,刘相国.抑制ZmCol3基因表达调控玉米开花期[J].玉米科学,2017,25(6):28-33. |
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| 作者姓名： | 贾伟 尹悦佳 柳青 刘洋 李楠 果天宇 郝东云 刘相国 |
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| 作者单位： | 吉林农业大学生命科学学院, 长春 130118;吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林农业大学生命科学学院, 长春 130118;吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林农业大学生命科学学院, 长春 130118;吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033 |
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| 基金项目： | 国家自然科学基金面上项目（31771879）、吉林省农业科技创新工程杰出青年项目（CXGC2017JQ014） |
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| 摘 要： | 采用ZmCol3基因RNAi载体构建、农杆菌介导玉米遗传转化、转基因材料开花期表型鉴定等研究方法,评估抑制ZmCol3基因表达对玉米开花期的影响。转基因玉米基因组PCR结果证实,人工合成RNAi片段已成功整合到玉米基因组中。qRT-PCR结果表明,在不同转基因玉米株系中ZmCol3基因表达受到不同程度的抑制。温室转基因玉米开花期相关性状调查结果表明,抑制ZmCol3表达,可以将玉米抽雄、散粉和吐丝时间提前2~3 d。研究结果证实,ZmCol3具有调控开花期的生物学功能,抑制该基因表达进而缩短玉米开花期可以作为行之有效的方法应用到玉米熟期改良研究中。
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| 关 键 词： | 玉米 RNA干扰 ZmCol3 开花期 |
| 收稿时间： | 2017/11/13 0:00:00 |
Regulation of Maize Flowering Time by Down-regulated ZmCol3 Gene Expression |
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| JIA Wei,YIN Yue-ji,LIU Qing,LIU Yang,LI Nan,GUO Tian-yu,HAO Dong-yun and LIU Xiang-guo.Regulation of Maize Flowering Time by Down-regulated ZmCol3 Gene Expression[J].Journal of Maize Sciences,2017,25(6):28-33. |
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| Authors: | JIA Wei YIN Yue-ji LIU Qing LIU Yang LI Nan GUO Tian-yu HAO Dong-yun and LIU Xiang-guo |
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| Institution: | College of Life Sciences, Jilin Agricultural University, Changchun 130118;Jilin Provincial Key Laboratory of Agricultural Biotechnology, lnstitute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,Jilin Provincial Key Laboratory of Agricultural Biotechnology, lnstitute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,Jilin Provincial Key Laboratory of Agricultural Biotechnology, lnstitute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,Jilin Provincial Key Laboratory of Agricultural Biotechnology, lnstitute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,Jilin Provincial Key Laboratory of Agricultural Biotechnology, lnstitute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,College of Life Sciences, Jilin Agricultural University, Changchun 130118;Jilin Provincial Key Laboratory of Agricultural Biotechnology, lnstitute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,College of Life Sciences, Jilin Agricultural University, Changchun 130118;Jilin Provincial Key Laboratory of Agricultural Biotechnology, lnstitute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China and Jilin Provincial Key Laboratory of Agricultural Biotechnology, lnstitute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China |
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| Abstract: | ZmCol3 gene RNAi vector construction, agrobacterium mediated genetic transformation and phenotypic identification of transgenic maize at flowering stage were used to evaluate the effect on ZmCol3 inhibited expression at maize flowering stage. PCR showed that RNAi vector had been successfully integrated into the maize genome; qRT-PCR results showed that ZmCol3 gene expression in different transgenic lines was inhibited in different degrees. Traits in the survey at flowering stage showed that inhibition of the ZmCol3 expression, could make the corn tassel, pollen and silk 2-3 days ahead of time. These results confirm that ZmCol3 has biological function in regulating flowering stage, inhibiting the ZmCol3 expression and shortening the flowering period of maize can be used as an effective method to improve the maturity. |
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| Keywords: | Maize RNAi ZmCol3 Flowering time |
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