金刚砂辅助农杆菌进行玉米基因转化研究

对超声波处理方式进行改进,在超声波处理时,加入金刚砂以辅助EPSPS基因转导到玉米幼胚中。结果表明,800粒处理种子播种出苗93株,玉米苗经大田glyphosate筛选有27株具有抗性,进行PCR电泳检测,显示23株阳性,初步证明EPSPS基因已经转化到玉米植株中,转化率为29%。金刚砂辅助处理玉米基因转化具有可行性,利于生产实际操作。

Transgenic Corn Research by Emery-assisted Agrobacterium-Mediated Transformation

The ultrasonication-assisted Agrobacterium treatment method was modified with the addition of emery to increase the treatment intensity to the apical meristem cells of germinating seeds. Ninety three plants from 800 control seeds were sprayed three times with glyphosate solution, and 27 of them survived, their leaves were taken with a PCR electrophoresis test and 23 shown positive, which suggested that epsps gene was introduced into the corn cultivar, and the transformation rate was 29%. Finally, which has been shown that the method has the advantages of circumventing the tedious tissue culture procedures and being easy to apply.