| 农杆菌介导玉米幼胚遗传转化体系的研究 |
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| 引用本文： | 王宏伟,梁业红,史振声,张世煌.农杆菌介导玉米幼胚遗传转化体系的研究[J].玉米科学,2011,19(3):73-75. |
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| 作者姓名： | 王宏伟 梁业红 史振声 张世煌 |
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| 作者单位： | 1. 沈阳农业大学特种玉米研究所,沈阳,110866
2. 中国农业科学院作物科学研究所,北京,100081 |
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| 基金项目： | 国家转基因重大专项(2009ZX08009-111B)、辽宁省玉米育种工程技术分中心项目(2006402031)、沈阳市特种玉米遗传生理重点实验室项目(1062107-1-00) |
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| 摘 要： | 以携带pCAMBIA1301(含GUS基因)质粒的EHA105菌株侵染玉米优良自交系齐319的幼胚,培养3 d后,借助GUS基因的瞬时表达率,对影响农杆菌介导玉米幼胚转化的部分因素进行优化。结果表明,农杆菌侵染液最佳浓度OD600为0.4～0.6,最适合用于转化的幼胚大小为直径1.5 mm,最佳侵染时间为20 min,GUS瞬时表达率为23.46%。
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| 关 键 词： | 玉米 幼胚 农杆菌介导 遗传工程 |
| 收稿时间： | 4/8/2010 12:00:00 AM |
Study on Agrobacterium Tumefaciens Mediated Transformation of Maize Immature Embryos |
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| WANG Hong-wei,LIANG Ye-hong,SHI Zhen-sheng,ZHANG Shi-huang,GE Yun-xia.Study on Agrobacterium Tumefaciens Mediated Transformation of Maize Immature Embryos[J].Journal of Maize Sciences,2011,19(3):73-75. |
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| Authors: | WANG Hong-wei LIANG Ye-hong SHI Zhen-sheng ZHANG Shi-huang GE Yun-xia |
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| Institution: | WANG Hong-wei1,LIANG Ye-hong2,SHI Zhen-sheng1,ZHANG Shi-huang2,GE Yun-xia1(1.Institute of Special Corn,Shenyang Agricultural University,Shenyang 110161,2.Institute of Crop Science,Chinese Agricultural Sciences,Beijing 100081,China) |
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| Abstract: | An optimized transformation system is developed for maize elite inbred lines using Agrobacterium mediated gene transfer by studying various important factors that affect transformation efficiency. The system was performed by transient expression of GUS gene after explants were placed on cultivation medium for 3 days. The study with immature embryos of Qi319 showed that the optimized Agribacterium suspension was as following, the concentration of Agrobacterium in the inoculation medium was adjusted to OD600=0.4-0.6. The size of the embryos was about 1.5 mm in length. The time of transformation was about 20 min. GUS transient expression efficiency was 23.46%. |
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| Keywords: | Maize Immature embryos Agrobacterium tumefaciens mediated Genetic engineering |
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