| 一株对玉米螟高毒力的苏云金芽孢杆菌的鉴定及其杀虫活性 |
| |
| 引用本文： | 赵宇,刘宏伟,路杨,王一平,李娜,关宇,张正坤,杜茜.一株对玉米螟高毒力的苏云金芽孢杆菌的鉴定及其杀虫活性[J].玉米科学,2017,25(6):148-152. |
| |
| 作者姓名： | 赵宇 刘宏伟 路杨 王一平 李娜 关宇 张正坤 杜茜 |
| |
| 作者单位： | 吉林省农业科学院/农业部东北作物有害生物综合治理重点实验室, 长春 130033,吉林省农业科学院/农业部东北作物有害生物综合治理重点实验室, 长春 130033,吉林省农业科学院/农业部东北作物有害生物综合治理重点实验室, 长春 130033,哈尔滨师范大学生命科学与技术学院, 哈尔滨 150025,哈尔滨师范大学生命科学与技术学院, 哈尔滨 150025,哈尔滨师范大学生命科学与技术学院, 哈尔滨 150025,吉林省农业科学院/农业部东北作物有害生物综合治理重点实验室, 长春 130033,吉林省农业科学院/农业部东北作物有害生物综合治理重点实验室, 长春 130033 |
| |
| 基金项目： | 吉林省科技攻关计划重点科技攻关项目（20150204015NY）、吉林省农业科技创新工程重大项目（CXGC2017ZD006） |
| |
| 摘 要： | 从田间土壤中收集并分离获得野生菌株,进行菌体、伴孢晶体形态学观察及分子生物学鉴定,测定其对玉米螟及水稻二化螟的室内生物活性。结果表明,该菌株革兰氏染色阳性,产生的伴孢晶体形状多为不规则形。16S rDNA序列与苏云金芽孢杆菌属的BMB 171同源性达99%,鉴定为苏云金芽孢杆菌,命名为JN002。该菌株对亚洲玉米螟具有较高毒力,对水稻二化螟毒性不及玉米螟,对黏虫几乎无毒力。该菌株是1株对亚洲玉米螟具有较强毒力的苏云金芽孢杆菌新菌株,同时也具备防治水稻二化螟的潜力。
|
| 关 键 词： | 玉米 亚洲玉米螟 水稻二化螟 菌株鉴定 微生物杀虫剂 |
| 收稿时间： | 2017/8/22 0:00:00 |
Identification and Toxicity Evaluation of A New Bacillus thuringiensis Strain |
| |
| ZHAO Yu,LIU Hong-wei,LU Yang,WANG Yi-ping,LI N,GUAN Yu,ZHANG Zheng-kun and DU Qian.Identification and Toxicity Evaluation of A New Bacillus thuringiensis Strain[J].Journal of Maize Sciences,2017,25(6):148-152. |
| |
| Authors: | ZHAO Yu LIU Hong-wei LU Yang WANG Yi-ping LI N GUAN Yu ZHANG Zheng-kun and DU Qian |
| |
| Institution: | Jilin Academy of Agricultural Sciences, Key laboratory of Integrated Pest Management on Crops in Northeast Ministry of Agriculture, Changchun 130033,Jilin Academy of Agricultural Sciences, Key laboratory of Integrated Pest Management on Crops in Northeast Ministry of Agriculture, Changchun 130033,Jilin Academy of Agricultural Sciences, Key laboratory of Integrated Pest Management on Crops in Northeast Ministry of Agriculture, Changchun 130033,College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China,College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China,College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China,Jilin Academy of Agricultural Sciences, Key laboratory of Integrated Pest Management on Crops in Northeast Ministry of Agriculture, Changchun 130033 and Jilin Academy of Agricultural Sciences, Key laboratory of Integrated Pest Management on Crops in Northeast Ministry of Agriculture, Changchun 130033 |
| |
| Abstract: | A new wild strain was obtained from the experimental field by chessboard sampling method, its gram and parasporal crystals morphology and molecular biology identification were undertaken, furthermore, its biological activity against O. furnacalis and Chilo suppressalis were detected. The results showed that it was a gram-positive strain and produced irregular parasporal crystals mostly. The result of 16S rDNA analysis revealed that the strain was homologous to B. thuringiensis strain BMB 171 with 99% similarity. All these results suggested that the strain was a new member of B. thuringiensis and designated as B. thuringiensis JN002. It gave a high toxicity against O. fumacalis, and lower of that against C. suppressalis, while showed extremely low activity against Mythimna separate. It demonstrated that JN002 was a new strain for O. furnacalis control, and also was potential for C. suppressalis control. |
| |
| Keywords: | Maize Ostrinia furnacalis Guenée Chilo suppressalis Strain identification Microbial pesticide |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《玉米科学》浏览原始摘要信息 |
| 点击此处可从《玉米科学》下载免费的PDF全文 |
|
