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西北地区糯玉米自交系遗传多样性研究
引用本文:陈婧,李建平.西北地区糯玉米自交系遗传多样性研究[J].玉米科学,2014,22(3):29-35.
作者姓名:陈婧  李建平
作者单位:宁夏大学农学院, 银川750021;宁夏大学农学院, 银川750021
基金项目:宁夏大学博士科研启动基金项目(BQD2012008)
摘    要:采用32对SSR引物对西北地区的40份糯玉米的遗传多样性进行分析,研究西北地区糯玉米种质资源的遗传多样性。结果表明,供试材料中检测出152个等位基因变异,每对引物可检测等位基因2~9个,平均4.75个。SSR的引物的PIC值介于0.303~0.862之间,平均多态性信息量为0.632。聚类分析表明,在遗传相似性系数(GS)0.662水平上40个自交系可聚成5类,主坐标分析将其分为4类9组。2种分类方法获得的结果基本吻合,主坐标分析能更为真实反映40个糯玉米自交系间的亲缘关系。

关 键 词:糯玉米  自交系  遗传多样性  西北地区
收稿时间:2013/6/18 0:00:00

Genetic Diversity of Waxy Corn Inbred Lines in Northwest China
CHEN Jing and LI Jian-ping.Genetic Diversity of Waxy Corn Inbred Lines in Northwest China[J].Journal of Maize Sciences,2014,22(3):29-35.
Authors:CHEN Jing and LI Jian-ping
Institution:Agricultural College, Ningxia University, Yinchuan 750021, China;Agricultural College, Ningxia University, Yinchuan 750021, China
Abstract:The genetic diversity of 40 waxy corn inbred lines in Northwest China was analyzed by 32 simple sequence repeats(SSR) marks. The results showed that the SSR primers giving stable amplified band pattern detected 152 alleles among the lines tested. The average number of alleles per SSR locus was 4.75 with a range from 2-9. The value of polymorphism information content(PIC) for each SSR locus varied from 0.303 to 0.862 with average of 0.632. Forty inbred lines were classified into 5 groups at the level of GS=0.662 by cluster analysis, while 4 groups and 9 subgroups could be sorted by principal coordinate analysis. The results from systematic cluster analysis and principal coordinate analysis were similar. However, more authentic genetic relationship among the 40 waxy corn inbred lines varieties could be provided by the principal coordinate analysis.
Keywords:Waxy corn  Inbred line  Genetic diversity  Northwest China
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