一个新的玉米雄性不育材料的发现及其初步遗传分析

研究从农杆菌介导法所获得的转Bt基因再生植株后代中得到大量不育株。用受体自交系18(红)授粉保种后,获得稳定不育的株系。通过两年、两点的种植,并结合与18(红)的多代回交以及与多个自交系的测交鉴定,对其遗传行为进行了初步研究。研究结果表明,其不育性状表现稳定,呈现出质核互作雄性不育的遗传特点。PCR检测及田间鉴定表明,外源基因是否整合到受体基因组内与不育性状的形成没有直接关系。雄性不育突变体的出现可能是组织培养过程所形成的体细胞克隆变异。

Discovery and Genetic Study of New Male Sterile in Maize

In this research,we obtained a great deal of sterile plants from the offspring of transgenic Bt maize by agrobacterium.Obtained stable male sterile inbreds when this materials were pollinated by acceptor inbred lines of "18 red".The sterile stability was observed in 2 locations,for 2 years and 3 generations.In order to analyse the kind of male sterile,many normal inbred lines were used to cross with this mutant.The main results are as the follow: the male sterile mutant is stable,inheritable in different years and different locations.It attributes to cytoplasmic male sterility.The appearance of male sterile mutant is the conclusion of somaclona variation.