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农杆菌介导向玉米茎尖导入HAL1基因的初步研究
引用本文:孙传波,曲文利,姜志磊,韦正乙,麻鹏达,李晓辉,张举仁,刘德璞,郝东云,袁 英.农杆菌介导向玉米茎尖导入HAL1基因的初步研究[J].玉米科学,2009,17(6):32-34.
作者姓名:孙传波  曲文利  姜志磊  韦正乙  麻鹏达  李晓辉  张举仁  刘德璞  郝东云  袁 英
作者单位:1. 吉林省农业科学院生物技术研究中心,长春,130033
2. 山东大学生命科学学院,济南,250100
基金项目:吉林省科技发展计划项目(20076010)
摘    要:选用玉米优良自交系郑58的茎尖为受体,研究以玉米茎尖为受体进行农杆菌转化体系的可行性。用农杆菌介导法将耐盐碱基因HAL1转入玉米中,获得70株转化苗,经过300 mg/L的除草剂(Basta)筛选,共获得9株转基因植株。进一步进行 PCR检测,其中6株表现阳性,转化率达8.57%。初步证明外源基因已经整合到玉米基因组中,以玉米茎尖作为受体孢子体的转化系统可用于基因转化。

关 键 词:玉米  茎尖  农杆菌介导    HAL1基因
收稿时间:2009/8/12 0:00:00

Preliminary Study on Agrobacterium Tumefaciens Mediated Transformation of HAL1 Gene into Shoot Apical Point of Maize
SUN Chuan-bo,QU Wen-li,ZHANG Ju-ren,HAO Dong-yun,YUAN Ying,et al..Preliminary Study on Agrobacterium Tumefaciens Mediated Transformation of HAL1 Gene into Shoot Apical Point of Maize[J].Journal of Maize Sciences,2009,17(6):32-34.
Authors:SUN Chuan-bo  QU Wen-li  ZHANG Ju-ren  HAO Dong-yun  YUAN Ying  
Abstract:The HAL1 gene was transferred into the shoot apical point of maize inbred line zheng 58 by Agrobacterium tumefaciens mediated transformation to study the feasibility of this system. Nine transgenic plants were obtained after selecting with 300 mg/L Basta and 6 of them were confirmed positive by PCR amplification. The frequency of transformation reached about 8.57%. The study showed that the foreign gene has integrated into maize genome and the shoot apical point of maize as receipt sporophyte can be used for transformation.
Keywords:Maize  Shoot apical point  A grobacterium tumefaciens mediated  HAL1
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