| 利用超声波处理玉米种子芽对农杆菌转化的影响 |
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| 引用本文： | 吕孟雨,董福双,张俊敏,王海波.利用超声波处理玉米种子芽对农杆菌转化的影响[J].玉米科学,2018,26(5):54-57. |
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| 作者姓名： | 吕孟雨 董福双 张俊敏 王海波 |
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| 作者单位： | 河北省农林科学院遗传生理研究所/河北省植物转基因中心 |
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| 基金项目： | 河北省自然科学基金项目（C2017301071）、河北省财政项目（2017039339） |
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| 摘 要： | 为提高玉米种子芽生长点的转化效率,研究利用超声波处理玉米种子芽时间对农杆菌转化效果的影响。结果表明,利用超声波频率45 KHZ、超声波功率50 W的超声波仪处理10~15 min,有利于提高农杆菌对玉米的转化效果。表明利用超声波处理玉米芽是一种好的受体处理方法。
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| 关 键 词： | 玉米 种子芽 超声波 农杆菌 转基因 |
| 收稿时间： | 2018/2/11 0:00:00 |
Effect of Maize Seed Bud Treated by Ultrasonic during Agrobacterium Transformation |
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| L&#; Meng-yu,DONG Fu-shuang,ZHANG Jun-min and WANG Hai-bo.Effect of Maize Seed Bud Treated by Ultrasonic during Agrobacterium Transformation[J].Journal of Maize Sciences,2018,26(5):54-57. |
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| Authors: | L&#; Meng-yu DONG Fu-shuang ZHANG Jun-min and WANG Hai-bo |
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| Institution: | Plant Genetic Engineering Center of Hebei Province, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China,Plant Genetic Engineering Center of Hebei Province, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China,Plant Genetic Engineering Center of Hebei Province, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China and Plant Genetic Engineering Center of Hebei Province, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China |
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| Abstract: | In this study, we used ultrasonic to treat the growing point of maize seed bud for different times, aiming to improve its transformation efficiency. The efficiency of maize growing point transformation mediated by Agrobacterium was significantly improved when it was treated by ultrasonic with frequency of 45 kHz and power of 50 W for 10-15 min. Thus, it is a good way for improving transformation efficiency mediated by Agrobacterium to treat receptor with ultrasonic. |
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| Keywords: | Maize Seed bud Ultrasonic Agrobacterium Transgene |
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