| 耐盐碱转基因玉米的获得及其抗性分析 |
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| 引用本文： | 郭嘉,孙传波,杨向东,袁英.耐盐碱转基因玉米的获得及其抗性分析[J].玉米科学,2016,24(6):24-29. |
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| 作者姓名： | 郭嘉 孙传波 杨向东 袁英 |
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| 作者单位： | 吉林省农业科学院农业生物技术研究所, 长春 130033;吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所, 长春 130033,吉林省农业科学院农业生物技术研究所, 长春 130033;吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所, 长春 130033;吉林省农业生物技术重点实验室, 长春 130033 |
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| 基金项目： | 吉林省农业科技创新工程[吉财教（2012）1072] |
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| 摘 要： | 依据Gen Bank数据库中发表的拟南芥DREB基因序列进行优化,人工合成DREB基因序列,构建植物表达载体,通过农杆菌介导法将DREB基因转入玉米自交系Hi II中,获得转DREB基因玉米材料,经过分子检测获得4个阳性转化事件。在不同浓度水平的Na Cl溶液(40、80、120 mmol/L)和Na_2CO_3溶液(10、20、30 mmol/L)胁迫下,研究其耐盐碱性。结果表明,DREB基因已经整合到玉米基因组中,转DREB基因玉米的耐盐碱性获得显著提高,80 mmol/L的Na Cl溶液和20 mmol/L Na_2CO_3溶液可以作为转DREB基因玉米的耐盐碱分析条件。
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| 关 键 词： | 转基因玉米 NaCl Na2CO3 耐盐碱 DREB基因 |
| 收稿时间： | 2016/7/14 0:00:00 |
Production and Analysis of Saline-alkali Tolerant Transgenic Maize |
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| GUO Ji,SUN Chuan-bo,YANG Xiang-dong and YUAN Ying.Production and Analysis of Saline-alkali Tolerant Transgenic Maize[J].Journal of Maize Sciences,2016,24(6):24-29. |
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| Authors: | GUO Ji SUN Chuan-bo YANG Xiang-dong and YUAN Ying |
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| Institution: | Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033;Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, China,Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033,Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033;Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, China and Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033;Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, China |
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| Abstract: | Based on the published in GenBank database of arabidopsis thaliana DREB gene sequence optimization, synthetic DREB gene sequences, the construction of plant expression vector, the DREB gene was transferred into maize inbred line HiII by Agrobacterium tumefaciens mediated transformation. Get fourpositive transformation events. Saline-alkali tolerance of transgenic maize carrying DREB was analyzed, under different saline-stress conditions(NaCl 40, 80, 120 mmol/L) and different alkali-stress conditions(Na2CO3 10, 20, 30 mmol/L). The main results were summarized that the DREB was integrated into maize genome and the saline-alkali tolerance was improved. 80 mmol/L NaCl and 20 mmol/L Na2CO3 were the appropriate concentration in saline-alkali tolerance analysis on transgenic maize. |
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| Keywords: | Transgenic maize NaCl Na2CO3 Saline-alkali stress DREB |
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