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玉米磷素相关根系性状Meta-QTL及候选基因发掘
引用本文:刘秀林,苗丽丽,薛永国,高明杰,蒲国锋,苏俊.玉米磷素相关根系性状Meta-QTL及候选基因发掘[J].玉米科学,2016,24(6):30-35.
作者姓名:刘秀林  苗丽丽  薛永国  高明杰  蒲国锋  苏俊
作者单位:黑龙江省农业科学院大豆研究所, 哈尔滨 150086;黑龙江省农业科学院博士后科研工作站, 哈尔滨 150086,东北农业大学农学院, 哈尔滨 150021,黑龙江省农业科学院大豆研究所, 哈尔滨 150086,黑龙江省农业科学院大豆研究所, 哈尔滨 150086,黑龙江省农业科学院大豆研究所, 哈尔滨 150086,黑龙江省农业科学院, 哈尔滨 150086
摘    要:以玉米高密度遗传连锁图谱IBM2 2008 Neighbors为参考图谱,收集来自不同实验中的175个玉米磷素相关根系数量性状位点(QTL)信息,利用Biomercator 2.1软件,构建玉米磷相关根系QTL整合图谱。采用元分析技术,在10条染色体上发掘出26个与磷相关根系性状"一致性"QTL(MQTL),图距范围在0.8~14.3 c M之间,除第2、5、8号染色体外,其余染色体上均检测到2~4个MQTL。根据MQTL区间两端标记在玉米物理图谱Ref Gen_v2上的位置,将MQTL进行物理图谱定位,单个MQTL所在物理图谱上的图距范围在0.1~9.1 Mb。在MQTL 1、2、4、8、11、12、15、18、20、22中检测到15个与磷素吸收利用以及根系性状的候选基因。

关 键 词:玉米  磷素  根系  元分析  候选基因
收稿时间:5/6/2016 12:00:00 AM

Analysis of Meta-QTL and Candidate Genes Related to Phosphrous Related Root Traits in Maize
LIU Xiu-lin,MIAO Li-li,XUE Yong-guo,GAO Ming-jie,PU Guo-feng and SU Jun.Analysis of Meta-QTL and Candidate Genes Related to Phosphrous Related Root Traits in Maize[J].Journal of Maize Sciences,2016,24(6):30-35.
Authors:LIU Xiu-lin  MIAO Li-li  XUE Yong-guo  GAO Ming-jie  PU Guo-feng and SU Jun
Institution:Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086;Post-doctoral Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin 150086,Northeast Agricultural University, Harbin 150021,Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086,Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086,Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086 and Heilongjiang Academy of Agricultural Science, Harbin 150086, China
Abstract:In order to unlock the full potential of the information contained in these independent experiments, one hundred seventy-five phosphrous related QTL in maize were collected from maize genomic database(maizeGDB) and were compiled to construct the integration QTL map for phosphrous related root traits in maize(Zea mays L.). The high-scale genetic linkage map of maize IBM2 2008 Neighbors was used as reference map and the map were constructed with BioMercator 2.1 software based on the method of meta-analysis. Twenty six consistent MQTL for maize were found. The range of MQTL interval distance was 0.8-14.3 cM, 2-4 MQTL were found in each chromosomes except in 2, 5 and 8 chromosomes. According the both end of marker, the physical position of MQTL in Ref Gen_v2 of chromosomes was found. The range of each MQTL was 0.1-9.1 Mb, a total of 15 candidate genes in the consensus QTL region were discovered.
Keywords:Maize  Phosphorus  Root trait  Meta-analysis  Candidate gene  
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