| 利用BSA法发掘玉米抗灰斑病主效QTL |
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| 引用本文： | 吕香玲,郑克志,李元,宋茂兴,闫伟,张旷野,李凤海,史振声.利用BSA法发掘玉米抗灰斑病主效QTL[J].玉米科学,2015,23(5):16-20. |
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| 作者姓名： | 吕香玲 郑克志 李元 宋茂兴 闫伟 张旷野 李凤海 史振声 |
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| 作者单位： | 沈阳农业大学特种玉米研究所, 沈阳 110866,沈阳农业大学特种玉米研究所, 沈阳 110866,沈阳农业大学特种玉米研究所, 沈阳 110866,沈阳农业大学特种玉米研究所, 沈阳 110866,沈阳农业大学特种玉米研究所, 沈阳 110866,沈阳农业大学特种玉米研究所, 沈阳 110866,沈阳农业大学特种玉米研究所, 沈阳 110866,沈阳农业大学特种玉米研究所, 沈阳 110866 |
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| 基金项目： | 国家自然科学基金(31301322)、辽宁省自然科学基金(2013020073)、辽宁省教育厅一般项目(L2012244)、辽宁省科特派项目(2014215031) |
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| 摘 要： | 以玉米高抗灰斑病自交系齐319与高感病自交系Ye478构建的RILS(重组自交系)为试材,通过两年田间表型鉴定,选取极端表型家系高抗16个,高感15个,利用SSR分子标记,并结合群体分离分析方法(BSA)筛选玉米抗灰斑病连锁标记并进行基因定位。结果表明,在玉米第1连锁群上检测到1个主效抗病基因位点(QTL),与两侧的分子标记umc2614和bnlg1803遗传图距分别为4.74 c M和3.78 c M,该抗病基因位点可解释40.9%的表型变异率,抗病基因来源于齐319,加性效应达到了-7.817 5。
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| 关 键 词： | 玉米 灰斑病 群体分离分析方法(BSA) SSR标记 基因定位 |
| 收稿时间： | 2015/6/30 0:00:00 |
Identification of Major QTL for Maize Gray Leaf Spot Resistance by BSA Method |
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| L&#; Xiang-ling,ZHENG Ke-zhi,Li Yuan,SONG Mao-xing,YAN Wei,ZHANG Kuang-ye,LI Feng-hai and SHI Zhen-sheng.Identification of Major QTL for Maize Gray Leaf Spot Resistance by BSA Method[J].Journal of Maize Sciences,2015,23(5):16-20. |
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| Authors: | L&#; Xiang-ling ZHENG Ke-zhi Li Yuan SONG Mao-xing YAN Wei ZHANG Kuang-ye LI Feng-hai and SHI Zhen-sheng |
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| Institution: | Institute of Specialty Maize, Shenyang Agricultural University, Shenyang 110866, China,Institute of Specialty Maize, Shenyang Agricultural University, Shenyang 110866, China,Institute of Specialty Maize, Shenyang Agricultural University, Shenyang 110866, China,Institute of Specialty Maize, Shenyang Agricultural University, Shenyang 110866, China,Institute of Specialty Maize, Shenyang Agricultural University, Shenyang 110866, China,Institute of Specialty Maize, Shenyang Agricultural University, Shenyang 110866, China,Institute of Specialty Maize, Shenyang Agricultural University, Shenyang 110866, China and Institute of Specialty Maize, Shenyang Agricultural University, Shenyang 110866, China |
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| Abstract: | A maize RIL population derived from a cross between highly resistant gray leaf spot inbred line Qi319 and highly susceptible gray leaf spot inbred line Ye478 was field-tested. Sixteen highly resistant families and fifteen highly susceptible families were selected respectively to develop two DNA bulks based on the phenotypic identification results through artificial inoculation gray leaf spot pathogen in two years. The bulks are screened for differences using simple sequence repeat(SSR) markers., and the markers were used for linkage analysis to locate the major Quantitative Trait Loci QTL). The results showed that one major QTL was detected accurately on chromosome 1. The linkage distance to umc2614 and bnlg1803 are 4.74 cM and 3.78 cM respectively. The QTL explained 40.9% of the phenotypic variation, and additive genetic effects -7.817 5. |
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| Keywords: | Maize Gray leaf spot(GLS) Bulked segregant analysis(BSA) SSR marker Gene location |
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