| 基于RNA-Seq技术的o2胚乳差异表达分析 |
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| 引用本文： | 朱慧,周志强,张东民,周昱婕,张晓星,刘红军,宋丽雅,李明顺.基于RNA-Seq技术的o2胚乳差异表达分析[J].玉米科学,2019,27(5):28-33. |
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| 作者姓名： | 朱慧 周志强 张东民 周昱婕 张晓星 刘红军 宋丽雅 李明顺 |
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| 作者单位： | 中国农业科学院作物科学研究所/农业部遗传育种重点实验室;山东农业大学/作物学国家重点实验室;北京工商大学/北京市植物资源研究开发重点实验室 |
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| 基金项目： | 东华北区早熟抗逆耐密适宜机械化玉米新品种培育项目（2017YFD0101106）、国家现代农业产业技术体系建设专项（CAR02-01）、北京市自然科学基金资助项目（31401390） |
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| 摘 要： | Opaque2(O2)转录因子在玉米胚乳的发育进程中起重要作用。o2突变体中赖氨酸含量增加,玉米品质提高,但在很多农艺性状上表现较差。以授粉后18 d的玉米胚乳为材料,使用RNA测序(RNA-Seq)技术筛选到3 051个差异表达基因(DEGs)。通过GO和KEGG富集分析发现,DEGs覆盖了物质跨膜运输、次生代谢物的生物合成、植物激素信号转导、脂质代谢、氮代谢等多方面的功能。由此推测,O2是玉米胚乳发育过程中多种代谢途径的关键性调节因子。
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| 关 键 词： | 玉米 O2 胚乳 RNA-Seq 育种 |
| 收稿时间： | 2018/9/11 0:00:00 |
Differential Expression Analysis of o2 Endosperm Based on RNA-Seq Technology |
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| ZHU Hui,ZHOU Zhi-qiang,ZHANG Dong-min,ZHOU Yu-jie,ZHANG Xiao-xing,LIU Hong-jun,SONG Li-ya and LI Ming-shun.Differential Expression Analysis of o2 Endosperm Based on RNA-Seq Technology[J].Journal of Maize Sciences,2019,27(5):28-33. |
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| Authors: | ZHU Hui ZHOU Zhi-qiang ZHANG Dong-min ZHOU Yu-jie ZHANG Xiao-xing LIU Hong-jun SONG Li-ya and LI Ming-shun |
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| Institution: | Department of Crop Genetics and Breeding, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081,Department of Crop Genetics and Breeding, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081,Department of Crop Genetics and Breeding, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081,Department of Crop Genetics and Breeding, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081,Department of Crop Genetics and Breeding, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081,State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai''an 271001,Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100048, China and Department of Crop Genetics and Breeding, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081 |
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| Abstract: | |
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| Keywords: | Maize O2 Endosperm RNA-Seq Breeding |
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