| 郑58/opaque2近等基因系中opaque2基因突变位点分析与高效分子标记开发 |
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| 引用本文： | 韩小花,鲁晓民,周波,魏良明,王延召,张前进,朱卫红,魏昕,郭书磊,刘康,郭金生,王振华,张新.郑58/opaque2近等基因系中opaque2基因突变位点分析与高效分子标记开发[J].玉米科学,2019,27(2):61-68. |
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| 作者姓名： | 韩小花 鲁晓民 周波 魏良明 王延召 张前进 朱卫红 魏昕 郭书磊 刘康 郭金生 王振华 张新 |
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| 作者单位： | 河南省农业科学院粮食作物研究所/河南省玉米重点实验室 |
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| 基金项目： | 国家重点研发计划（2016YFD0101205-4）、国家重点研发计划项目（2016YFD0300309/03）、河南省科技攻关项目（172102110075）、河南省基础与前沿技术研究计划项目（162300410178） |
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| 摘 要： | opaque2突变体材料是最常用的高赖氨酸玉米供体。对已获得的郑58/o2近等基因系研究表明,胚乳发育不同时期22-kDα-醇溶蛋白的积累均明显低于郑58。荧光定量PCR结果表明,α-醇溶蛋白家族基因Z1A、Z1B、Z1C和Z1D的表达均显著低于郑58。郑58/o2胚乳发育不同时期Opaque2基因均正常表达。测序分析发现,郑58/o2中o2基因ATG后713 bp处缺失10个碱基,ORF预测Opaque2蛋白翻译提前终止。针对突变缺失位点开发基因内分子标记o2-indel-1,利用该标记进行回交转育,结果表明,o2-indel-1与o2突变表型完全连锁,错选率为0。opaque2基因突变位点的解析有助于高效分子标记的开发,有效降低错选率,提高优质蛋白鲜食玉米多基因聚合育种的选择效率。
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| 关 键 词： | 玉米 醇溶蛋白 opaque2 基因突变 |
Analysis of the Mutation Site of opaque2 Gene in Near-isogenic Line Zheng 58/Opaque-2 and Development of Functional Molecular Marker |
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| HAN Xiao-hu,LU Xiao-min,ZHOU Bo,WEI Liang-ming,WANG Yan-zhao,ZHANG Qian-jin,ZHU Wei-hong,WEI Xin,GUO Shu-lei,LIU Kang,GUO Jin-sheng,WANG Zhen-hua and ZHANG Xin.Analysis of the Mutation Site of opaque2 Gene in Near-isogenic Line Zheng 58/Opaque-2 and Development of Functional Molecular Marker[J].Journal of Maize Sciences,2019,27(2):61-68. |
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| Authors: | HAN Xiao-hu LU Xiao-min ZHOU Bo WEI Liang-ming WANG Yan-zhao ZHANG Qian-jin ZHU Wei-hong WEI Xin GUO Shu-lei LIU Kang GUO Jin-sheng WANG Zhen-hua and ZHANG Xin |
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| Institution: | The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China,The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China and The Cereal Crops Institute, Henan Academy of Agricultural Sciences/Henan Key Labortary of Maize Biology, Zhengzhou 450002, China |
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| Abstract: | |
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| Keywords: | Maize Zein opaque2 Gene mutation |
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