| 基于两种荧光毛细管电泳平台筛选评估玉米新型SSR引物 |
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| 引用本文： | 刘文彬,许理文,王凤格,赵久然,冯博,赵涵,吕远大,蔚荣海.基于两种荧光毛细管电泳平台筛选评估玉米新型SSR引物[J].玉米科学,2017,25(2):24-30. |
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| 作者姓名： | 刘文彬 许理文 王凤格 赵久然 冯博 赵涵 吕远大 蔚荣海 |
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| 作者单位： | 北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室, 北京 100097;吉林农业大学农学院, 长春 130118,北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室, 北京 100097,北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室, 北京 100097,北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室, 北京 100097,北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室, 北京 100097;吉林农业大学农学院, 长春 130118,江苏省农业科学院农业生物技术研究所, 南京 210014,江苏省农业科学院农业生物技术研究所, 南京 210014,吉林农业大学农学院, 长春 130118 |
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| 基金项目： | 国家科技支撑计划（2015BAD02B02）、北京市农林科学院院科技创新能力建设专项（KJCX20161501） |
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| 摘 要： | 以93份代表自交系及27套包括杂交种及双亲在内的三联体材料为试材,分别用Qiagen和ABI3730两种DNA分析仪,从111对新型玉米SSR引物中筛选出多态性高、稳定性好、适于自动化分型的引物9对,确定1套适合SSR引物评估筛选的方案,可以为今后引物设计至评估入选提供高效的筛选方案提供参考。
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| 关 键 词： | 玉米 DNA分析仪 新型SSR引物 引物评估筛选 入选率 |
| 收稿时间： | 2016/10/31 0:00:00 |
Evaluating and Screening New Maize SSR Primer Based on Two Kinds of Fluorescent Capillary Eelectrophoresis Platform |
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| LIU Wen-bin,XU Li-wen,WANG Feng-ge,ZHAO Jiu-ran,FENG Bo,ZHAO Han,L&#; Yuan-da and YU Rong-hai.Evaluating and Screening New Maize SSR Primer Based on Two Kinds of Fluorescent Capillary Eelectrophoresis Platform[J].Journal of Maize Sciences,2017,25(2):24-30. |
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| Authors: | LIU Wen-bin XU Li-wen WANG Feng-ge ZHAO Jiu-ran FENG Bo ZHAO Han L&#; Yuan-da and YU Rong-hai |
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| Institution: | Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences, Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097;College of Agriculture, Jilin Agricultural University, Changchun 130118,Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences, Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097,Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences, Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097,Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences, Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097,Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences, Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097;College of Agriculture, Jilin Agricultural University, Changchun 130118,Institute of Agricultural Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China,Institute of Agricultural Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China and College of Agriculture, Jilin Agricultural University, Changchun 130118 |
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| Abstract: | Using the 93 inbred lines and 27 sets of mother-father-child trios containing hybrid and parents as the experimental material, separately using two kinds of DNA analyzer to screen primers about Qiagen and ABI3730, screening 9 pairs of primers with high polymorphism, good stability and suitable for automatic classification from 111 pairs of primers, determined a set of suitable for SSR primers screening evaluating scheme.in order to lay a foundation for provide high efficiency screening scheme about primers design, evaluate and choose in future. |
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| Keywords: | Maize DNA analyser New SSR primer Primers screening and evaluating Selected ratio |
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