利用EMS化学诱变改造玉米自交系的研究

选用生产上的玉米骨干自交系,使用不同浓度的甲基磺酸乙酯(EMS)处理其成熟花粉.处理后代采用系谱法种植;在后代群体选择早熟、晚熟和各种有利用价值的株型变异;通过M1、M2代两代田间选择和M3代田间鉴定, 从多个自交系中选出了已稳定的早熟、晚熟突变体和一个有利用价值的株型突变体;并得出浓度为0.667×10-3的EMS-石蜡油有利于早熟突变的发生,而大于10-3的EMS-石蜡油有利于晚熟突变的发生.由于所得自交系为原自交系的等基因突变体,因此该技术在育种实践和理论上具有重要意义。

Studies on Reform of Corn Inbred Line by EMS

The earlier matured mutant,the later matured mutant and other valuable mutant of plant type were produced by direct treating mature pollen of main inbred line of maize in field.The offspring treated were planted by pedigree method.Through M₁ and M₂ selection and M₃ evaluation in field,some mutants were stable.The results also indicated that the 0.667×10^-3treatment level of EMS attributed to the earlier matured mutant and the overl0^-3treatment level attribute to later matured mutant.The mutants were isogonic lines of original inbred because of point mutation.Thus the technique has a great meaninh breeding practice and theoretical research..