玉米幼胚组织培养体系优化

以5个骨干玉米自交系幼胚为材料, 研究幼胚大小、2, 4-D浓度、KT浓度对幼胚愈伤组织诱导及分化的影响。结果表明, 5个骨干玉米自交系均可诱导出愈伤组织, 但不同条件下诱导率存在显著差异。最适幼胚大小为2.0～3.0 mm, 因基因型不同而有所变化;2, 4-D浓度在1.0～3.0 mg/L有利于愈伤组织诱导, 同一材料不同2, 4-D浓度对胚性愈伤组织诱导率的影响具有显著差异, 不能以初级愈伤诱导率高低作为选择胚性愈伤诱导率的标准;KT可显著提高愈伤组织的分化率, 随着KT浓度的增加分化率呈先上升后下降的趋势, 最适KT浓度为1.0～2.5 mg/L。

Optimization of Immature Embryo Tissue Culture in Maize

Immature embryos of 5 maize lines were used to study the size of immature embryos, 2, 4-D concentration, KT concentration on the callus induction and callus differentiation. The research showed that callus could be induced from all the tested inbred lines, but there was significant difference among the embryonic callus induction for different condition. The choice size of 2.0-3.0 mm young embryos was explants to be advantageous to obtain perfect callus, but it will be different under the change of genotypes. 1.0-3.0 mg/L 2, 4-D could induce maize immature embryos to produce callus more effectively, primary callus could not be the selection criteria of embryogenic callus. KT was able to promote the maize callus differentiation rate, with the increasing of the concentration of KT the differentiation rate was along the trade of first increased and then decreased. Optimum KT concentration was 1.0-2.5 mg/L, it was different for different genotypes.