正交试验法在玉米组织培养中的应用

利用正交试验研究了培养基、2,4-D、蔗糖、脯氨酸、水解酪蛋白(CH)5个因素及组合对玉米自交系4112和P1幼胚愈伤组织诱导的影响,探讨了正交设计应用于玉米培养基筛选的可能性.结果表明:1)对4112诱导胚性愈伤的最优组合是8114培养基、2,4-D 4 mg/L、蔗糖80g/L、CH 500mg/L,对P1最优组合是蔗糖N6培养基、2,4-D 1 mg/L、蔗糖20 g/L、脯氨酸1 400mg/L、CH 500mg/L;2)不同玉米自交系的幼胚需要不同的糖浓度和培养基;3)适宜的2,4-D浓度可提高愈伤组织的诱导率;4)脯氨酸和水解酪蛋白对愈伤组织的形成有一定作用。

Application of Orthogonal Experiment in Tissue Culture of Maize

The difference of callus initiation of maize inbred line4112and P1from young embryos on different media were compared.Experiments were conducted by using the orthogonal experiment design.The type of media,2,4-D,sucrose,proline and CH were used to compare their effects on callus initiation from young embryos.The pos-sibility of application of the orthogonal experiment method in tissue culture of maize inbred line was discussed.Re-sults showed that(1)the best combination for4112was8114culture media,2,4-D4mg /L,sucrose80g /L,CH500mg /L,for P1was N6media,2,4-D1mg /L?sucrose20g /L?proline1400mg /L,CH500mg /L;(2)the embryos of different maize inbred line species demanded different concentration of sucrose for callus initiation;(3)the suitable concentration of2,4-D was favorable for promoting the yield of callus;(4)it was quite useful for callus initiation to use proline and CH.