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玉米单倍体自然加倍若干问题探讨
引用本文:吴鹏昊,任姣姣,田小龙,刘晨旭,刘文欣,陈绍江.玉米单倍体自然加倍若干问题探讨[J].玉米科学,2016,24(4):7-11.
作者姓名:吴鹏昊  任姣姣  田小龙  刘晨旭  刘文欣  陈绍江
作者单位:中国农业大学/国家玉米改良中心/北京作物遗传改良重点实验室, 北京 100193;新疆农业大学农学院, 乌鲁木齐 830052,中国农业大学/国家玉米改良中心/北京作物遗传改良重点实验室, 北京 100193,中国农业大学/国家玉米改良中心/北京作物遗传改良重点实验室, 北京 100193,中国农业大学/国家玉米改良中心/北京作物遗传改良重点实验室, 北京 100193,中国农业大学/国家玉米改良中心/北京作物遗传改良重点实验室, 北京 100193,中国农业大学/国家玉米改良中心/北京作物遗传改良重点实验室, 北京 100193
基金项目:国家自然科学基金(31560392)、现代农业产业技术体系(CARS-02-09)、“十三五”国家重点研发计划“玉米杂种优势利用技术与强优势杂交种创制”
摘    要:玉米单倍体加倍是DH育种中的关键技术环节之一,玉米单倍体雄穗自然加倍能力较低,限制了DH育种技术在商业育种中的大规模推广,对玉米雄穗自然加倍遗传特性的研究将有助于DH技术在育种中的进一步应用。本研究通过对多个不同遗传背景的单倍体雄穗自然加倍能力进行严格统计和差异比较,结果表明,单倍体雄穗育性恢复主要受核基因的控制,不受细胞质基因的调控。诱导率对单倍体雄穗自然加倍能力没有显著影响,自交系来源的DH1经过诱导后的单倍体由于基因型没有变化,其自然加倍能力与自交系相比没有显著提高。

关 键 词:玉米  单倍体  自然加倍  遗传特性
收稿时间:2016/4/18 0:00:00

Genetic Studies on Haploid Spontaneous Doubling in Maize
WU Peng-hao,REN Jiao-jiao,TIAN Xiao-long,LIU Chen-xu,LIU Wen-xin and CHEN Shao-jiang.Genetic Studies on Haploid Spontaneous Doubling in Maize[J].Journal of Maize Sciences,2016,24(4):7-11.
Authors:WU Peng-hao  REN Jiao-jiao  TIAN Xiao-long  LIU Chen-xu  LIU Wen-xin and CHEN Shao-jiang
Institution:College of Agriculture and biotechnology/National Maize Improvement Center of China/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193;College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China,College of Agriculture and biotechnology/National Maize Improvement Center of China/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193,College of Agriculture and biotechnology/National Maize Improvement Center of China/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193,College of Agriculture and biotechnology/National Maize Improvement Center of China/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193,College of Agriculture and biotechnology/National Maize Improvement Center of China/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193 and College of Agriculture and biotechnology/National Maize Improvement Center of China/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193
Abstract:Haploid spontaneous doubling in maize is one of the key technological steps in DH breeding, however, average rate of male fertility is very low, which limits the broadly commercial application of DH technology. Therefore, so genetic study of maize haploid spontaneous doubling could facilitate further application of DH technology in crop breeding procedure. In this study, haploids with different genetic background were investigated with their male fertility and compared with each other, the result showed that haploid spontaneous doubling of male fertility is mainly controlled by genes in nucleus, but not genes in the cytoplasm; the haploid induction rate has little effect on haploid male fertility. There is also no significant difference of haploid spontaneous doubling between haploids from DH1 and their corresponded inbred because of unchangeable genotypes.
Keywords:Maize  Haploid  Spontaneous doubling  Genetic characteristic
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