| 基因枪共转化法获得玉米转基因植株的研究 |
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| 引用本文： | 郭嘉,孙传波,陶蕊,李海华,孟凡梅,曲文利,郝东云,袁英.基因枪共转化法获得玉米转基因植株的研究[J].玉米科学,2012,20(1):44-47. |
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| 作者姓名： | 郭嘉 孙传波 陶蕊 李海华 孟凡梅 曲文利 郝东云 袁英 |
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| 作者单位： | 吉林省农业科学院生物技术研究中心,长春,130033 |
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| 基金项目： | 养分高效利用转基因玉米新品种培育(2008ZX08003-005) |
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| 摘 要： | 通过基因枪共转化方法将磷高效利用phy基因、钾高效利用AlHAK1基因和筛选标记bar基因等外源基因导入玉米自交系H99的1 500块愈伤组织中。经PCR分析,获得79株阳性植株,其中53株为AlHAK1阳性,49株为phy阳性,23株为AlHAK1和phy阳性,单基因转化率为5.27%,双基因转化率为1.53%,共转化整合比率达到29.11%。
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| 关 键 词： | 玉米 自交系 基因枪 共转化 |
| 收稿时间： | 3/8/2011 12:00:00 AM |
Study on the Development of Transgenic Maize Plants by Particle-bombardment Co-transformation |
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| GUO Ji,SUN Chuan-bo,TAO Rui.Study on the Development of Transgenic Maize Plants by Particle-bombardment Co-transformation[J].Journal of Maize Sciences,2012,20(1):44-47. |
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| Authors: | GUO Ji SUN Chuan-bo TAO Rui |
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| Institution: | GUO Jia,SUN Chuan-bo,TAO Rui,et al.(Biotechnology Research Centre,Jilin Academy of Agricultural Sciences,Changchun 130033,China) |
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| Abstract: | The phy gene, AlHAK1 gene and bar gene were transferred into 1 500 maize embryonic calli of maize inbred line H99 by particle-bombardment co-transformation. Seventy-nine positive plants were gained by PCR analysis, in which 53 plants showed AlHAK1 positive, 49 plants showed phy positive, 23 plants showed AlHAK1 and phy positive. The transformation frequency of single-gene and bi-genes was 5.27% and 1.53%, respectively. The co-transformation frequency reached about 29.11%. |
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| Keywords: | Maize Inbred line Particle-bombardment Co-transformation |
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