Biological evaluation of iron availability from pre-adolescent diets by using anaemic rats

The bioavailability of iron from habitually consumed diet by pre-adolescent children of low socio-economic group and the diet supplemented with additional 8 g of protein supplied by 34 g legumes (Green gram and Moth bean 1:1) or 195 ml of buffalo's milk to per day diet was studied in anaemic rats using haemoglobin regeneration method. The bioavailability of iron for haemoglobin regeneration relative to FeSO₄ was 69.5 percent from milk supplemented diet as compared to 47.5 percent from the diet normally consumed by pre-adolescent children of low socio-economic group while legume supplementation to the diet showed no improvement.