Effects of Pressurized Low Polarity Water Extraction Parameters on Antioxidant Properties and Composition of Cow Cockle Seed Extracts |
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Authors: | Özlem Güçlü-Üstünda? G Mazza |
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Institution: | (1) Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, Summerland, BC, Canada, V0H 1Z0 |
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Abstract: | Antioxidant activity of pressurized low polarity water (PLPW) extracts of cow cockle seed and extraction residues were determined
using DPPH, ABTS, and FRAP assays. The effect of extraction conditions (temperature (125, 150 and 175 °C) and time) on the
antioxidant activity and the relationship amongst the antioxidant activity and extract composition (total phenolics and saponin
content) were determined. The antioxidant activity of PLPW extracts increased with extraction temperature. Increasing activity
with time was also observed at 175 °C. PLPW extraction residues had the highest activity suggesting antioxidant compounds
were not completely extracted by PLPW. Antioxidant activity correlated well with total phenolics content of samples (R
2 ≥ 0.94), however no correlation was observed with the saponin content. A strong correlation was observed between the antioxidant
activity values obtained using different methods (R
2 ≥ 0.94). These results point to the potential of PLPW extraction as a method to modify the activity of biological materials
for the production of customized extracts. |
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Keywords: | Vaccaria segetalis Saponaria vaccaria Cow cockle seed Phenolics Saponins Subcritical water extraction Antioxidant activity DPPH ABTS FRAP |
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