Effect of efficient pectinolytic bacterial isolates on retting and fibre quality of jute |
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Authors: | Biswapriya Das Kalyan ChakrabartiSagarmoy Ghosh Bijan MajumdarSudipta Tripathi Ashis Chakraborty |
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Institution: | a Department of Agricultural Chemistry and Soil Science, Institute of Agricultural Science, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India b Department of Microbiology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India c Central Research Institute for Jute & Allied Fibres, Indian Council of Agricultural Research, Barrackpore, Kolkata 700120, West Bengal, India d Department of Agronomy, Bidhan Chandra Krishi Viswavidyalaya, West Bengal 741252, India |
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Abstract: | Four bacterial strains with high balanced polygalacturonase (PG) and pectin lyase (PNL), and xylanase with minimal cellulase activity were used in jute retting. The 16S rDNA analysis revealed that the organisms were: Bacillus sp. L6 (GQ891097), Bacillus pumilus strain EK-17 (GQ891098), B. pumilus strain Geo-03-422 (GQ891103) and B. pumilus strain IK-MB12-518F (GQ891105). The microorganisms in different combinations of consortia showed synergistic effect resulting in increased PG (35.52-46.61 IU/g cell wet weight), PNL (39.79-72.12 U/ml), xylanase (0.705-0.840 μmol/ml/min) and little cellulase (0-0.153 μmol/ml/min) activities. The organisms in different combinations of consortia reduced the retting period from 11 to 13 days as compared to 19 days in the control. Microbial inoculation produced remarkable improvement in jute fibre strength (26.62-28.91 g/tex) and fineness (2.76-2.92 tex) over control. The pH of the post-retting water samples became acidic, and the electrical conductivity (Ec), chemical oxygen demand (COD) and hardness increased. The organisms could be adopted in industrial application for extraction of jute fibre. |
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Keywords: | Polygalacturonase Pectin lyase Xylanase 16S rDNA analysis Fibre strength Fineness |
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