Role of exogenous phytohormones on growth and plumbagin accumulation in Plumbago indica hairy roots and conservation of elite root clones via synthetic seeds |
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Authors: | Moumita Gangopadhyay Saikat DewanjeeDipjyoti Chakraborty Sabita Bhattacharya |
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Institution: | a Medicinal Plant Laboratory, Department of Botany, Bose Institute, 93/1 APC Road, Kolkata 700009, India b Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Raja SC Mallik Road, Kolkata 700032, India c Plant Molecular & Cellular Genetics, Bose Institute, P 1/12, CIT Road, Scheme VIIM, Kolkata 700054, India |
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Abstract: | The present study describes the role of different exogenous hormones on morphology and plumbagin production in Plumbago indica hairy roots. It was also aimed to conserve elite root clones via synthetic seed technology. Insertion of rolB gene in transformed roots was confirmed by polymerase chain reaction followed by southern blot analysis. Hairy roots were treated with single or in combination of different phytohormones viz. IAA, IBA, 2, 4-D, NAA, BAP, GA3 and ABA. Cultures incubated with GA3 (0.5 mg l−1) yielded highest root growth due to formation of profuse lateral branching while NAA (0.5 mg l−1) treatment caused highest plumbagin accumulation. Cultures incubated with 2, 4-D exhibited highest inhibitory effect in terms of both root growth and plumbagin production. All phytohormones were found to be effective at lower concentration. In combinatorial study, GA3 + NAA (0.5 mg l−1, each) was found optimum for root biomass and plumbagin production at earlier stage of culture. Different combinations of auxins and BAP induced different morphologies ranging from reduction of lateral branching to rapid disorganization of root matrix. The combinations of ABA and selected auxins were not found promising at any of selected concentration. Based on the effect of exogenous hormones on hairy root culture, elite root clones were selected and encapsulated with sodium alginate matrix. Uniform shaped alginate coated synthetic seeds were conserved up to 6 months exhibited high regeneration potential without disturbing plumbagin content. |
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Keywords: | IAA indole-3-acetic acid IBA indole-3-butyric acid NAA 1-naphtheleneacetic acid 2 4-D 2 4-dichlorophenoxyacetic acid BAP 6-benzylaminopurine GA3 gibberellic acid ABA abscisic acid PCR polymerase chain reaction HPLC High Pressure Liquid Chromatography Fw fresh root biomass Dw dry root biomass |
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