| 华南地区大豆根内拮抗菌AF-67筛选与鉴定 |
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| 引用本文： | 区伟佳,王荣,李敏清,袁英英,李华兴.华南地区大豆根内拮抗菌AF-67筛选与鉴定[J].大豆通报,2010(2):18-21. |
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| 作者姓名： | 区伟佳 王荣 李敏清 袁英英 李华兴 |
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| 作者单位： | 华南农业大学资源环境学院; |
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| 基金项目： | 国家自然科学基金项目(40971155);;广东省“十一五”攻关项目(2006B20301050);;广东省教育部产学研结合项目(2009B090300330);;广东省农业厅科技项目(粤农土肥(2004)28号) |
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| 摘 要： | 筛选生防菌共436株,有生防性能71株,占16.3%,抑菌效果较好8株,占1.8%,能快速高命中率的获得具有防病效果好、抑菌谱广的生防菌株。内生菌AF-67与Fusarium oxysporum共培养时,能使其菌丝体及其分生孢子生长明显畸变。经16S rDNA基因序列分析,AF-67被初步鉴定为Bacillus amyloliquefaciens。
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| 关 键 词： | 大豆根腐病 根内拮抗菌 筛选 鉴定 16SrDNA |
Isolation and identificantion of antifungal entophytic bacteria AF-67 from soybean root in South China |
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| Qu Weijia,Wang Rong,Li Minqing,et al..Isolation and identificantion of antifungal entophytic bacteria AF-67 from soybean root in South China[J].Soybean Bulletin,2010(2):18-21. |
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| Authors: | Qu Weijia Wang Rong Li Minqing |
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| Abstract: | In this study,the improved traditional screening methods could quickly obtain strains which showed antagonistic activity against soybean root rot and broad antagonistic spectrum.The activity of 436 strains assayed,16.3% of them showed antagonistic activity against F.oxysporum f.sp.soybean and one of them,strain AF67 isolated from soybean roots was the best.Co-culture with Fusarium oxysporum,mycelium and conidia were evident distortion.Based on the sequence of 16S rDNA,strain AF67 was identified as Bacillus ... |
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| Keywords: | Soybean root rot Antifungal Entophytic Bacteria Isolation Characterization 16S rDNA |
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