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| 基因枪法将GmDREB基因导入大豆的研究 | |
| 引用本文： | 王萍,郭永来,高世庆,马有志,程宪国,李文滨.基因枪法将GmDREB基因导入大豆的研究[J].大豆科学,2007,26(3):315-318. |
| 作者姓名： | 王萍 郭永来 高世庆 马有志 程宪国 李文滨 |
| 作者单位： | 1. 淮海工学院海洋学院,连云港,222005 2. 长春龙洋大豆生物蛋白股份有限公司,长春,130041 3. 中国农业科学院作物栽培育种研究所,北京,100081 4. 中国农业科学院土肥所,北京,100081 5. 东北农业大学大豆研究所/国家教育部大豆生物学重点实验室,哈尔滨,150030 |
| 基金项目： | 教育部大豆生物学重点实验室资助项目（SB06A07）;国家植物转基因研究与产业化专项（JY03-A-18） |
| 摘 要： | 测定大豆体细胞胚对PPT的基础抗性,用基因枪法将pRdGM-bar质粒转化大豆体细胞胚,得到抗性体细胞胚和抗性植株,经PCR、PCRsouthern检测,证明CmDREB基因转入到大豆中. |
| 关 键 词： | 大豆 基因枪GmDREB基因 体细胞胚 |
| 文章编号： | 1000-9841（2007）03-0315-04 |
| 修稿时间： | 2007-02-25 |
TRANSFORMING GmDREB GENE INTO SOYBEAN VIA PARTICLE BOMBARDMENT |
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| WANG Ping,GUO Yong-lai,GAO Shi-qing,MA You-zhi,CHENG Xian-guo,LI Wen-bin.TRANSFORMING GmDREB GENE INTO SOYBEAN VIA PARTICLE BOMBARDMENT[J].Soybean Science,2007,26(3):315-318. | |
| Authors: | WANG Ping GUO Yong-lai GAO Shi-qing MA You-zhi CHENG Xian-guo LI Wen-bin |
| Institution: | 1. School of Marine Science and Techology , Huaihai Institute of Technology, Lianyungang 222005; 2. Changchun Long Yang Soybean Biologic Albumen Co. Ltd, Changchun 130062; 3. Institute of Crop Breeding and Cultivation ,Chinese Academy of Agricultural Sciences ,Beijing 100081 ;4. Institute of Soil and Fertility, Chinese Academy of Agricultural Sciences, Beijing 100081 ; 5. Soybaen Research Institute Key Laboratory of Soybean Biology in Chinese Ministry of Education Northeast Agricultural University, Harbin 150030 |
| Abstract: | The basis resistance of somatic embryo in soybean to PPT was tested. Plasmid pRdGM -bar was transformed into somatic embryo of soybean via particle bombardment. The resistant somatic embryos and plantlets were obtained. It was demonstrated that the GmDREB gene had been transformed into soybean by polymerase chain reaction and PCR-Southern detection. |
| Keywords: | Soybean Particle bombardment GmDREB gene Somatic embryo |
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