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| 大豆体细胞胚再生植株的研究 | |
| 引用本文： | 王晓春,刘尚前,王罡,季静,王萍.大豆体细胞胚再生植株的研究[J].大豆科学,2006,25(2):149-152. |
| 作者姓名： | 王晓春 刘尚前 王罡 季静 王萍 |
| 作者单位： | 1. 天津大学农业与生物工程学院,天津,300072;河北北方学院南校区农业科学系,河北,宣化,075131 2. 河北北方学院南校区农业科学系,河北,宣化,075131 3. 天津大学农业与生物工程学院,天津,300072 4. 淮海工学院海洋学院,连云港,222005 |
| 基金项目： | "国家植物转基因技术研究开发与中试基地建设"专项课题 |
| 摘 要： | 以球形期大豆体细胞胚为材料,对其进行继代增殖、萌发及再生植株的研究.结果表明,大豆体细胞胚每隔15-20天分割成3mm左右的小块在MS培养基附加10-20 mg/L 2,4-D的固体培养基以及弱光条件下,可以继续增殖,继代增殖能力强,扩繁系数为3n(n为继代培养次数);大豆体细胞胚性组织先在含有活性碳的培养基上培养,能提高其萌发率以及正常胚率,1个月以后转移到无活性碳的培养基,又能提高其再生速度以及再生率. |
| 关 键 词： | 大豆 体细胞胚 再生植株 |
| 文章编号： | 1000-9841（2006）02-0149-04 |
| 收稿时间： | 2005-09-22 |
| 修稿时间： | 2005-09-22 |
A STUDY OF SOYBEAN PLANT REGENERATION VIA SOMATIC TISSUE CULTURES |
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| Wang Xiaochun,Liu Shangqian,Wang Gang,Ji Jing,Wang Ping.A STUDY OF SOYBEAN PLANT REGENERATION VIA SOMATIC TISSUE CULTURES[J].Soybean Science,2006,25(2):149-152. | |
| Authors: | Wang Xiaochun Liu Shangqian Wang Gang Ji Jing Wang Ping |
| Institution: | 1. Tian jin University of Agricultural and Biological Engineering Tianjin 300072; 2. Department of Agriculture sciences of Hebei North University, Xuanhua 075131; 3. Huahai Institute of Technology, Marine School ,Lianyungang , 222005 |
| Abstract: | The system of tissue culture were studied about sparal somatic embryos of soybean,the media about the subculture,germination and regeneration were established.The result showed that the media for somatic embryogenesis proliferation was MS with 10~20mg/L 2,4-D and weak light when they were divided into part somatic embryos with dismater 3mm every 15~20 day.The subculture coefficiency reached 3~n,("n"was the times of proliferation culture),the germination frequency and normal somatic embryos frequency of soybean increased when active carbon was applied;when they were cultured one month under this condition,again they were transferred into the media without active carbon,the regeneration frequency and the regeneration speed of soybean increased. |
| Keywords: | Soybean Somatic embryos of soybean Regeneration plants |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |