| EMS诱发大豆“南农94-16”突变体库的扩建及部分突变体的SSR分析 |
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| 引用本文： | 陈远东,喻德跃.EMS诱发大豆“南农94-16”突变体库的扩建及部分突变体的SSR分析[J].大豆科学,2009,28(4). |
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| 作者姓名： | 陈远东 喻德跃 |
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| 作者单位： | 南京农业大学大豆研究所国家大豆改良中心,作物遗传与种质创新国家重点实验室,江苏,南京,210095 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目，国家高技术研究发展计划(863计划)资助项目 |
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| 摘 要： | 甲基磺酸乙酯(EMS)是一种稳定、高效的诱发点突变的诱变剂.采用EMS处理大豆"南农94-16"的种子,在初步构建大豆突变体库的基础上进一步扩建了大豆突变体库,获得95份茎、叶、花、种子等性状变异的突变材料.采用200对SSR标记分别对其中高蛋白、叶黄化等9个不同性状的突变体进行了鉴定.结果表明:与对照相比突变体发生了多位点的变异.构建的突变体库不但可以作为新的种质资源,而且可促进大豆功能基因组研究的开展.
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| 关 键 词： | 大豆 突变体 |
Construction of Mutant Pools for Soybean Nannong 94-16 Induced by EMS and Analysis of SSR Marker on Several Mutants |
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| CHEN Yuan-dong,YU De-yue.Construction of Mutant Pools for Soybean Nannong 94-16 Induced by EMS and Analysis of SSR Marker on Several Mutants[J].Soybean Science,2009,28(4). |
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| Authors: | CHEN Yuan-dong YU De-yue |
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| Institution: | CHEN Yuan-dong,YU De-yue(Soybean Research Institute,Nanjing Agricultural University,National Center for Soybean Improvement,State Key Laboratory of Crop Genetics , Germplasm Enhancement,Nanjing 210095,Jiangsu,China) |
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| Abstract: | EMS is a stable and effective mutagen.In this study,soybean mutant pools were constructed by treating the seeds of soybeanNannong 94-16with EMS.These pools include 95 mutants of stem,leaf,cotyledon,flower and seed.In addition,200 SSR primer pairs were used to characterize several mutants such as high-protein content mutant,revealing polymorphism between mutants and base materials.These mutant pools created forNannong 94-16will be valuable for the research in breeding and functional genomics soybean. |
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| Keywords: | EMS SSR |
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