大豆芽期和苗期耐盐性评价指标筛选

大豆耐盐性是决定盐碱地大豆产量的关键因素之一。为探究不同盐浓度对大豆芽期、苗期生长发育的影响,评价不同大豆品种对不同盐浓度的耐受能力,以合豆3号、徐豆14、中黄55和临豆10号大豆品种为材料,在芽期,设置50,75和100 mmol·L~(-1)的盐溶液处理,苗期设置100,200和250 mmol·L~(-1)盐浓度处理。记录大豆芽期生长指标和苗期形态生理指标,并以各指标耐盐指数隶属函数值、总隶属函数值为依据,对4个大豆品种芽期和苗期耐盐性进行比较及聚类分析。结果表明:芽期主根长、下胚轴长、根干重、含水量、鲜重、根鲜重、活力指数耐盐指数的隶属函数值与总耐盐指数隶属函数值的相关性均达到极显著水平,苗期根干重、根鲜重、MDA含量、株高的耐盐指数与总耐盐指数隶属函数值的相关性均达到显著水平。在不同时期和不同品种的盐耐受分析中,不同生育时期的大豆耐受性表现不同;芽期4个大豆品种耐盐程度大致相同。苗期徐豆14、临豆10号耐盐性较强,中黄55耐盐性中等,合豆3号耐盐性较弱。在耐盐指标筛选分析中,下胚轴长、根干重、含水量、鲜生物量、根鲜重和主根长的耐盐指数可作为大豆芽期耐盐鉴定指标,而MDA含量和株高的耐盐指数则可以作为大豆苗期耐盐鉴定指标。

Related Indices Selection of Soybean Salt Tolerance at Germination and Seedling Stages

Soybean salt tolerance is one of the key factors in improving soybean yield in saline soils.In this study,four cultivars of soybean(Hedou 3,Xudou 14,Zhonghuang 55,Lindou 10) were treated with different concentrations of saline solution at the germination stage(50,75 and 100 mmol·L-1) and the seedling stage (100,200 and 250 mmol·L-1).We examined some morphological and physiological indicators at three concentration levels in two stages and transfered these measurement indicators to the subjection values of salt tolerance index and the sum subjection value.Then we studied the salt tolerance of the 4 cultivars by comparing the differences of salt toxicity coefficients of subjection values at the stage of germination and seedling respectively.Finally,the cluster analysis of salt toxicity coefficients of multi-indices was carried out.The results showed that the subjection values of salt tolerance index of root length,hypocotyl length,dhy weight of root,water content,fresh biomass,fresh weight of root and vigor index were significantly correlated with SUM subjection values at germination stage.And the subjection values of salt tolerance index of MDA content,plant height,dry weight of root and fresh weight of root were significantly correlated with SUM subjection values at seedling stage.Clustering analysis showed different salt tolerance among different cultivars and growth stages.No similarity soybean varieties were clustered at germination stage using the eight subjection values of salt tolerance index among the four soybean varieties,which indicated similar level of salt tolerance.At seedling stage,the cluster analysis grouped the varieties into three clusters.The first group of salt-resistant soybean varieties were Xudou 14 and Lindou 10,the second was moderately salt-tolerant cultivar Zhonghuang 55 and the third group was salt-sensitive cultivar Hedou 3.In selection of salt tolerance index,hypocotyl length,dry weight of root,water content,fresh biomass,fresh weight of root and root length could be served as the indicators to assess the salt tolerance of the cultivars at germination stage,while plant height and MDA content could be used to evaluate the salt tolerance at seedling stage.