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利用注射叶片法快速鉴定大豆GmCRY1和GmCRY2基因功能
引用本文:秦正睿,孙磊,李宏宇.利用注射叶片法快速鉴定大豆GmCRY1和GmCRY2基因功能[J].大豆科学,2012,31(1):13-16.
作者姓名:秦正睿  孙磊  李宏宇
作者单位:1. 吉林大学植物科学学院,吉林长春130062;中国农业科学院作物科学研究所,北京100081
2. 中国农业科学院作物科学研究所,北京,100081
基金项目:转基因专项资助项目(2009ZX08004-010B,2008ZX08010-004);国家高技术研究发展计划资助项目(2006AA10Z107)
摘    要:将大豆隐花色素基因GmCRY1、GmCRY2构建在N端含有绿色荧光蛋白(GFP)的pEGAD载体上,并转化农杆菌EHA105菌株.将含有上述基因的EHA105菌液分别注射到大豆单叶及复叶叶片中,观察长日条件下(16 h光照/8h黑暗)和短日条件下(8h光照/16 h黑暗)注射植株的开花时间.结果叶片注射含GmCRY1基...

关 键 词:大豆  隐花色素  根癌农杆菌  开花时间

Rapid Identification of GmCRY1 and GmCRY2 Function by Leaf Injection in Soybean
QIN Zheng-rui , SUN Lei , LI Hong-yu.Rapid Identification of GmCRY1 and GmCRY2 Function by Leaf Injection in Soybean[J].Soybean Science,2012,31(1):13-16.
Authors:QIN Zheng-rui  SUN Lei  LI Hong-yu
Institution:1.College of Plant Science,Jilin University,Changchun 130062,Jilin;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 10081,China)
Abstract:Soybean(Glycine max)Cryptochrome 1(GmCRY1)and Cryptochrome 2(GmCRY2)genes were constructed into expression vector pEGAD,and injected into soybean leaves by Agrobacterium tumefaciens EHA105 mediated transformation.The Agrobacterium tumefaciens EHA105 with vector pEGAD was used as control.The RT-PCR method was carried on to detect if the GmCRY1 and GmCRY2 were transient expressed in the leaves.The transformed plants were grown in different photoperiod light conditions(SD:8 h light/16 h darkness;LD:16 h light/ 8 h darkness)and the flowering data was calculated to conclude the effect of transient expression of GmCRY1 and GmCRY2 on the flowering time in soybean.The results indicated that the transient expressed GmCRY1 in soybean flowered earlier in LD,and the GmCRY1 expression was detected in the injected leaves through RT-PCR analysis.Although the expression of GmCRY2 was detected,it had no effect on flowering time in both LD and SD conditions.This result confirmed that the leaves injection method can quickly identify the function of GmCRY1 and GmCRY2 genes on soybean flowering time.
Keywords:Soybean  Cryptochrome genes  Agrobacterium tumefaciens  Flowering time
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