大豆遗传转化技术研究进展

高效、稳定的遗传转化体系是开展大豆转基因育种及功能基因组学研究的重要前提。自1988年首次建立基因枪和农杆菌介导的大豆遗传转化技术以来,围绕影响大豆转化效率提高的各种因素,国内外学者对大豆转化方法进行了多方面的改进,包括在共培养基中添加抗氧化剂类化合物,选用强毒农杆菌菌株以及不同的外植体和筛选剂等。经过20多年的发展,大豆遗传转化效率不断提高,目前已报道的大豆转化效率最高可达32.6%。文章对近年来大豆遗传转化技术研究进展进行了综述,并对影响大豆转化效率的主要因素进行了探讨。

Recent Advances in Soybean Transformation

Efficient and stable transformation is a prerequisite to production of transgenic soybean and soybean functional genomics research.Since the successful transformation of the cultivated soybean by Agrobacterium-mediated and microprojectile bombardment methods independently in 1988,soybean transformation efficiency has been greatly improved by modification of many important factors affecting soybean transformation and regeneration,including addition of antioxidant compounds mixture in co-cultivation medium,utilization of super-virulent strains,different explants and selection agents,etc.,with the highest transformation efficiency of 32.6% obtained recently.In this paper,recent advances in soybean transformation were reviewed and some factors influencing transformation efficiency were also discussed.