| 应用大孔树脂纯化大豆异黄酮的工艺研究 |
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| 引用本文： | 方建,楚楚,程冬萍,黄六仔,颜继忠.应用大孔树脂纯化大豆异黄酮的工艺研究[J].大豆科学,2012,31(3):474-477. |
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| 作者姓名： | 方建 楚楚 程冬萍 黄六仔 颜继忠 |
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| 作者单位： | 1. 浙江工业大学药学院,浙江杭州,310014
2. 浙江惠松制药有限公司,浙江杭州,310018 |
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| 基金项目： | 浙江省面向社会发展科技计划项目 |
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| 摘 要： | 先用聚醚砜有机膜对豆粕乙醇提取液进行超滤,然后用D101大孔树脂纯化,研究大豆异黄酮的精制工艺.结果表明:用孔径5000D的有机膜超滤处理豆粕乙醇提取液,能够去除其中56.1％的杂质.用D101大孔树脂纯化,采用上样液浓度为0.750 mg·mL -1大豆异黄酮溶液,流速为1.0 BV·h-1,依次用去离子水、1.0 ...
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| 关 键 词： | 大豆异黄酮 超滤 大孔树脂 |
Purification of Soybean Isoflavones by Macroporous Resin |
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| FANG Jian
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CHU Chu
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CHENG Dong-ping
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HUANG Liu-zai
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YAN Ji-zhong.Purification of Soybean Isoflavones by Macroporous Resin[J].Soybean Science,2012,31(3):474-477. |
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| Authors: | FANG Jian CHU Chu CHENG Dong-ping HUANG Liu-zai YAN Ji-zhong |
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| Institution: | 1(1.College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,Zhejiang;2.Zhejiang Huisong Pharmaceutical Co.,Ltd,Hangzhou 310018,Zhejiang,China) |
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| Abstract: | This paper studied the soybean isoflavones refining process by ultrafiltrating soybean meal extract with polyether sulphone organic membrane and then purified with D101 macroporous resin.The detailed soybean isoflavones refining process was studied and optimized conditions were obtained.The results showed that removal rate of the impurities was up to 56.1% by ultrafiltrating soybean ethanol extract with organic membrane of pore diameter 5 000 D.Purified by D101 macroporous resin,with 0.750 mg·mL-1 sample concentration and 1.0 BV·h-1 fluid samples rate,then successively rinsed with 1.0 BV deionized water,1.0 BV 10% ethanol and 2.0 BV 80% ethanol,purify of 41.2% soybean isoflavone products was obtained and the overall yield was 65.7%. |
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| Keywords: | Soybean isoflavones Ultrafiltration Macroporous resin |
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