| 高效液相色谱法( HPLC)测定大豆异黄酮含量的研究 |
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| 引用本文： | 张海军,苏连泰,李琳,刘雅婧,李晓薇,张艳,王英,李景文,王庆钰.高效液相色谱法( HPLC)测定大豆异黄酮含量的研究[J].大豆科学,2011,30(4):672-675,679. |
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| 作者姓名： | 张海军 苏连泰 李琳 刘雅婧 李晓薇 张艳 王英 李景文 王庆钰 |
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| 作者单位： | 吉林大学植物科学学院,吉林长春,130062 |
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| 基金项目： | 转基因生物新品种培育重大专项资助项目(2008ZX08004-003); 国家自然科学基金面上资助项目(30971808); 教育部新教师基金资助项目(3M2101936604); 吉林省科技发展计划重点资助项目(20080204) |
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| 摘 要： | 建立检测大豆异黄酮5种组分(染料木素、染料木苷、大豆苷元、大豆苷和黄豆黄苷)含量的高效液相色谱法,并且测定了大豆各组织和不同时期胚的异黄酮含量.色谱条件:Phenomenex C18色谱柱(150mm×4.6mm,5.0 μm)；流动相:甲醇-水(30∶70,v/v)；流速:1 mL· min-1,检测波长:254nm...
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| 关 键 词： | 大豆 高效液相色谱法 异黄酮含量 |
Determination of Soybean Isoflavones Content by High-Performance Liquid Chromatographic Method |
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| ZHANG Hai-jun,SU Lian-tai,LI Lin,LIU Ya-jing,LI Xiao-wei,ZHANG Yan,WANG Ying,LI Jing-wen,WANG Qing-yu.Determination of Soybean Isoflavones Content by High-Performance Liquid Chromatographic Method[J].Soybean Science,2011,30(4):672-675,679. |
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| Authors: | ZHANG Hai-jun SU Lian-tai LI Lin LIU Ya-jing LI Xiao-wei ZHANG Yan WANG Ying LI Jing-wen WANG Qing-yu |
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| Institution: | ZHANG Hai-jun,SU Lian-tai,LI Lin,LIU Ya-jing,LI Xiao-wei,ZHANG Yan,WANG Ying,LI Jing-wen,WANG Qing-yu(College of Plant Science,Jilin University,Changchun 130062,Jinlin,China) |
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| Abstract: | The five soybean isoflavones components including Genistein,Genistin,Daidein,Daidzin and Glycitin were determined and separated by the established HPLC method,and isoflavones in different tissues and different grow stages of embryo were measured.The HPLC condition we have established:an phenomenex C18column(150 mm×4.6 mm,5.0 μm)was applied;the mobile phase was MeOH ∶H2O=30 ∶70;the temperature of column was 40℃;the flow rate was 10 mL·min-1;detective wavelength was set at 254 nm.The peak area of five isoflavones had a good linearity with a correlation coefficient of 0.9994-0.9998,and the recoveries of standard spiling of isoflavones were in the range of 99.29%-100.76% with RSD less than 3%.This method was suitable for determination of isoflavones in soybean and other cereal products.The isoflavone content among soybean varieties was Jilin 32> GR8836>Jidadou 2> Pingan 8> Xiaoheidou>Jilin 47>Jilin 35>Jidadou 2,and soybean isoflavone content of Jilin 32 was that in seeds>60 d embryos>50 d embryos>40 d embryos>flowers>pods>20 d embryos>30 d embryos>leaves>roots>stems. |
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| Keywords: | Soybean(Glycine max) High-performance liquid chromatographic method(HPLC) Isoflavone content |
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