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| 高分辨率溶解曲线分析技术在大豆Ecotilling中的应用 | |
| 引用本文： | 焦丽,刘相国,杨春明,王虎义,郝东云,王庆钰.高分辨率溶解曲线分析技术在大豆Ecotilling中的应用[J].大豆科学,2011,30(5):843-846,850. |
| 作者姓名： | 焦丽 刘相国 杨春明 王虎义 郝东云 王庆钰 |
| 作者单位： | 1. 吉林大学植物科学学院,吉林长春,130062 2. 吉林省农业科学院生物技术研究中心,吉林长春,130124 3. 吉林省农业科学院大豆研究中心,吉林长春,130012 4. 吉林大学生命科学学院,吉林长春,130012 |
| 基金项目： | 吉林省科技发展计划重大资助项目(20086029) |
| 摘 要： | 将高分辨率溶解曲线(High Resolution Melting,HRM)技术运用在大豆Ecotilling中,对256份栽培大豆的大豆异黄酮合酶基因(IFS1)的外显子区域进行SNP筛查,并采用直接测序的方法对HRM检测结果进行验证.结果表明:模板均一化后的检测效果较未均一化的检测效果差异显著,野生型样本与待测样本... |
| 关 键 词： | 大豆 高分辨率溶解曲线分析技术 Ecotilling SNP |
Application of High Resolution Melting Analysis in Soybean Ecotilling |
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| JIA Li,LIU Xiang-guo,YANG Chun-ming,WANG Hu-yi,HAO Dong-yun,WANG Qing-yu.Application of High Resolution Melting Analysis in Soybean Ecotilling[J].Soybean Science,2011,30(5):843-846,850. | |
| Authors: | JIA Li LIU Xiang-guo YANG Chun-ming WANG Hu-yi HAO Dong-yun WANG Qing-yu |
| Institution: | JIAO Li1,LIU Xiang-guo2,YANG Chun-ming2,WANG Hu-yi3,HAO Dong-yun2,3,WANG Qing-yu1(1.College of Plant Science,Jilin University,Changchun 130062,2.Biotechnology Center,Jilin Academy of Agricultural Sciences,Changchun 130124,3.College of Life Science,Changchun 130012,Jilin,China) |
| Abstract: | Identification of allelic variants in natural populations by Ecotilling has became a rapid Single nucleotide polymorphisms(SNP) discovery method,but it is not convenient for detection.High resolution melting(HRM)analysis is a rapid,sensitive and high-throughput method that can be used in the examination of SNP.In this paper,HRM techniques was used in Ecotilling to detect the SNP of IFS1 exons in 256 soybean cultivars.The results of HRM was verified by direct sequencing.The effect of homogenization and non-h... |
| Keywords: | Soybean High resolution melting Ecotilling SNP |
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