| 大豆凝集素基因Le2克隆及转化 |
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| 引用本文： | 方标,王丕武,于雷,卢春蕾.大豆凝集素基因Le2克隆及转化[J].大豆科学,2011,30(4):706-709. |
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| 作者姓名： | 方标 王丕武 于雷 卢春蕾 |
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| 作者单位： | 吉林农业大学农学院,吉林长春,130118 |
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| 基金项目： | 国家转基因专项资助项目(2011ZX08004-004); 大学生科技创新基金资助项目 |
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| 摘 要： | 大豆凝集素对提高植物的抗虫性有重要作用.应用基因工程手段,克隆大豆凝集素基因Le2,构建植株表达载体pSy-Le2,将大豆凝集素Le2基因通过农杆菌介导法导入烟草中,经过筛选获得转基因植株,为进一步鉴定Le2的基因功能提供了试验基础.
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| 关 键 词： | 大豆凝集素 基因工程 抗虫性 农杆菌 烟草 |
Cloning and Transformation of Soybean Lectin Gene Le2 into Tobacco |
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| FANG Biao,WANG Pi-wu,YU Lei,LU Chun-lei.Cloning and Transformation of Soybean Lectin Gene Le2 into Tobacco[J].Soybean Science,2011,30(4):706-709. |
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| Authors: | FANG Biao WANG Pi-wu YU Lei LU Chun-lei |
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| Institution: | FANG Biao,WANG Pi-wu,YU Lei,LU Chun-lei(Biotechnology Center of Jilin Agricultural University,Changchun 130118,Jilin,China) |
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| Abstract: | Soybean lectin(Soybean agglutinin) is a major protein in soybean,which plays an important role in plant insects resistance as a main anti-nutritional factors.In this study,the soybean lectin gene Le2 had been cloned by genetic engineering technology,then constructed the recombinant plant expression vector pSy-Le2 and transformed it into tobacco by Agrobacterium mediated way.Transgenic plant had been selected,which supplied basic materials for further experiments. |
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| Keywords: | Soybean lectin Genetic engineering Insect resistance Agrobacterium Tobacco |
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