| 大豆DNA扩增片段长度多态性(AFLP)研究 |
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| 引用本文： | 田清震,盖钧镒,喻德跃,贾继增.大豆DNA扩增片段长度多态性(AFLP)研究[J].大豆科学,2000,19(3):210-217. |
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| 作者姓名： | 田清震 盖钧镒 喻德跃 贾继增 |
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| 作者单位： | 1. 南京农业大学大豆研究所 农业部国家大豆改良中心,南京 210095
2. 中国农科院品种资源研究所 农业部作物品种资源重点实验室,北京 100081 |
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| 基金项目： | 国家科技攻关项目;9601401064; |
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| 摘 要： | 本文研究ＡＦＬＰ技术在大豆上的应用,在改进大豆种子ＤＮＡ提取方法的基础上,比较同位素与银染检测方法的效果,筛选适宜大于大豆ＡＦＬＰ分析的酶切和引物组合,从而建立了适用于大豆指纹图谱快速鉴定的ＡＦＬＰ操作程序,并对我国野生大豆和栽培大豆代表性材料进行了ＡＦＬＰ分析。
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| 关 键 词： | 大豆 DNA提取 银染检测 遗传育种 AFLD技术 |
A STUDY ON AMPLIFIED FRAGMENT LENGTH POLYMORPHISM (AFLP) IN SOYBEAN |
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| Tian Qingzhen,Gai Junyi,Yu Deyue,Jia Jizeng.A STUDY ON AMPLIFIED FRAGMENT LENGTH POLYMORPHISM (AFLP) IN SOYBEAN[J].Soybean Science,2000,19(3):210-217. |
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| Authors: | Tian Qingzhen Gai Junyi Yu Deyue Jia Jizeng |
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| Institution: | Tian Qingzhen 1 Gai Junyi 1** Yu Deyue 1 Jia Jizeng 2 |
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| Abstract: | In the present paper,
improvement of the DNA extraction from dry soybean seed, comparison between the silver
staining and isotope labeling methods, and exploration of the endoenzyme and primer
combinations suitable for AFLP analysis in soybeans were presented. Based on this, a
simple and fast procedure suitable for soybean AFLP analysis was established,and the
representative accessions of G. soja and G. max from various ecologi- cal areas in China
were examined by using the suggested procedure. |
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| Keywords: | Soybean DNA extraction Silver-staining inspection AFLP (amplified fragment length polymorphism) * National Key Science and Technology Project No 960140106 ** Author for correspondence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
