| 大豆铁蛋白cDNA的克隆及植物表达载体的构建 |
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| 引用本文： | 王永斌,郭长虹,郭东林,蔡秋艺,张爽,李集临,林红.大豆铁蛋白cDNA的克隆及植物表达载体的构建[J].大豆科学,2006,25(4):454-457. |
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| 作者姓名： | 王永斌 郭长虹 郭东林 蔡秋艺 张爽 李集临 林红 |
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| 作者单位： | 1. 哈尔滨师范大学生物系,哈尔滨,150080
2. 牡丹江制药厂,牡丹江,157013 |
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| 基金项目： | 黑龙江自然科学基金C2005-07;哈尔滨市学科后备带头人项目2005AFXXJ016;黑龙江省青年基金QC05C60;黑龙江省教育厅海外学人科研项目1055HQ024 |
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| 摘 要： | 以大豆为材料，利用RT-PCR法扩增得到大小约750bp的片段，将这个片段连接到克隆载体pBlueskript SK＋上进行测序，结果证明，此片段就是大豆的铁蛋白基因。将此片段再正向插入到植物表达载体pBI121的CaMV35s启动子和NOS终止子之间，构建了植物表达载体pBIFe，并成功地将该载体导入根癌农杆菌EHA105。此项工作为获得表达铁蛋白的转基因植物奠定了基础。
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| 关 键 词： | 大豆铁蛋白 cDNA 克隆 植物表达载体 |
| 文章编号： | 1000-9841（2006）04-0454-04 |
| 收稿时间： | 2006-09-20 |
| 修稿时间： | 2006-09-20 |
CLONING OF SOYBEAN FERRITIN GENE AND CONSTRUCTION OF ITS PLANT EXPRESSION VECTOR |
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| Wang Yongbin,Guo Changhong,Guo Donglin,Cai Qiuyi,Zhang Shuang,Li Jilin,Lin Hong.CLONING OF SOYBEAN FERRITIN GENE AND CONSTRUCTION OF ITS PLANT EXPRESSION VECTOR[J].Soybean Science,2006,25(4):454-457. |
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| Authors: | Wang Yongbin Guo Changhong Guo Donglin Cai Qiuyi Zhang Shuang Li Jilin Lin Hong |
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| Institution: | 1. Department of Biology, Harbin Normal University, Harbin, 150080 ; 2. Mudanjiang Pharmaceutical Factory, Mudanjiang 157013 |
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| Abstract: | A 750bp fragment was amplified by RT-PCR from soybean.The PCR products were cloned into pBlueskript SK+ vector and named pFer after its sequences was confirmed.The soybean ferritin gene was subcloned into plant expression vector pBI121 and named PBI121Fer.Then the recombination plasmid were introduced into Agrobacterium EHA105.This work provides a foundation for transferring ferritin gene into plant by Agrobacterium mediated transformation. |
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| Keywords: | soybean ferritin cDNA cloning plant expression vector |
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