中国北方春大豆区大豆花叶病毒株系的鉴定与分布

从我国北方春大豆区的黑龙江、吉林、辽宁、内蒙、河北承德以及北京采集典型症状的大豆叶片病样,经Top-Crop离体叶单斑分离以及DAS-ELISA抗血清免疫鉴定,共获得112个SMV阳性反应分离物.根据112个分离物在合丰25、文丰5号、诱变30、8101、铁丰25、Davis、Buffalo、早熟18、广吉、科丰1号、齐黄22共11个鉴别寄主上的症状反应,将我国北方春大豆区的SMV分成8个株系群.各株系群所占比例分析表明,北方春大豆区以SC-11株系群为主,其次为SC-8,各占病样总数的比例分别为51.7%和17.9%,SC-11和SC-8分别在黑龙江和辽宁分布最广,占当地株系群的比例分别为67.8%和75.0%.与以往株系划分系统的初步比较表明,SC-11与东北原1号株系群,SC-12和SC-16与东北原2号株系群,SC-4、SC-7、SC-8、SC-13和SC-17与东北原3号株系群在鉴别寄主上的反应相似.SC-16和SC-17为2个新发现的株系群.

CLASSIFICATION AND DISTRIBUTION OF STRAIN GROUPS OF SOYBEAN MOSAIC VIRUS IN NORTHERN CHINA SPRING PLANTING SOYBEAN REGION

904 specimens of soybean mosaic virus(SMV) were collected from Heilongjiang,Jilin,Liaoning,Hebei,Neimeng and Beijing in Northern China Spring Planting Soybean Region.The symptomatology and serology were carried out to purify and detect SMV.From them,112 SMV isolates were obtained.Based on their response to 11 differential hosts such as Hefeng 25,Wenfeng 5,Youbian 30,8101,Tiefeng 25,Davis,Buffalo,Zaoshu 18,Kwanggyo,Kefeng 1 and Qihuang 22,112 SMV isolates were classified into 8 strain groups.Among them,SC-11 was the widest in Northern China Spring Planting Soybean Region with a proportion of 51.7%,and then SC-8,with a proportion of 17.9%.As far as the proportion of local SMV strain groups are concerned,the distributions of SC-11 and SC-8 were the widest in Heilongjiang with a proportion of 67.8% and in Liaoning with a proportion of 75.0%,respectively.The results of comparison of 11 SMV strain groups in present experiment with strain groups identified by Lu Wenqing showed that,SC11 was similar with No.1 strain groups,SC-12 and SC-16 were similar with No.2, SC-4,SC-7,SC-8,SC-13 and SC-17 were similar with No.3.SC-16 and SC-17 were new strain groups.