| 利用ADS-7大孔树脂分离纯化淡豆豉中异黄酮 |
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| 引用本文： | 牛丽颖,蔡广华,王鑫国.利用ADS-7大孔树脂分离纯化淡豆豉中异黄酮[J].大豆科学,2010,29(1). |
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| 作者姓名： | 牛丽颖 蔡广华 王鑫国 |
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| 作者单位： | 河北医科大学,中医学院,河北省实验动物重点实验室,河北,石家庄,050091;河北医科大学,中医学院,河北省实验动物重点实验室,河北,石家庄,050091;河北医科大学,中医学院,河北省实验动物重点实验室,河北,石家庄,050091 |
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| 基金项目： | 河北省自然基金资助项目(C2009001061) |
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| 摘 要： | 以染料木素为指标,采用紫外分光光度法测定异黄酮的含量,并利用ADS-7大孔树脂分离纯化淡豆豉中异黄酮。结果表明:该树脂分离淡豆豉异黄酮的工艺参数为:上样液浓度0.225 g.mL-1,pH值4.5,最大上样量150 mL,以4 BV.h-1的流速吸附,以8倍柱床体积的70%乙醇作为洗脱剂,洗脱流速4 BV.h-1,树脂可重复使用2次。经初步吸附分离,异黄酮得率达40%以上。
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| 关 键 词： | 淡豆豉 异黄酮 大孔吸附树脂 吸附 分离 |
Separation and Purification of Soybean Isoflavone in Semen Sojae Praeparatum by ADS-7 Macroporous Adsorptive Resins |
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| NIU Li-ying,CAI Guang-hua,WANG Xin-guo.Separation and Purification of Soybean Isoflavone in Semen Sojae Praeparatum by ADS-7 Macroporous Adsorptive Resins[J].Soybean Science,2010,29(1). |
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| Authors: | NIU Li-ying CAI Guang-hua WANG Xin-guo |
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| Institution: | College of Traditional Chinese Medicine;Hebei Medical University;Hebei Key Lab of Laboratory Animal Science;Shijiazhuang 050091;Hebei;China |
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| Abstract: | Use ultraviolet spectrophotometry to detect the content of soybean isoflavone,with the content of genistein as the index,to investigate the ADS-7 macroporous adsorptive resins for separation and purification of soybean isoflavone in the Semen Sojea Praeparatum. The optimized technological parameters of the ADS-7 macroporous adsorptive resins were as following:loading sample density 0.225 g · mL~(-1),pH 4.5,the maximum sample volume 150 mL,4 BV · h~(-1) flow rate to adsorption and the volume of 70%(v/v)ethanol as 8 BV with desorption-flow 4 BV · h~(-1). With these conditions,more than 40% of soybean isoflavone could be obtained from primary adsorption and purification,and the ADS-7 macroporous adsorptive resins can be used twice. |
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| Keywords: | Semen Sojea Praeparatum Soybean Isoflavone Macroporous Adsorptive Resins Adsorption Separation |
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