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6株生防细菌对大豆根腐病防治效果初步评价
引用本文:温广月,许艳丽,李春杰,陈冬.6株生防细菌对大豆根腐病防治效果初步评价[J].大豆科学,2005,24(2):121-125.
作者姓名:温广月  许艳丽  李春杰  陈冬
作者单位:中国科学院东北地理与农业生态研究所,哈尔滨,150040;东北农业大学,哈尔滨,150030;中国科学院东北地理与农业生态研究所,哈尔滨,150040
基金项目:国家科技攻关项目,中国科学院知识创新工程项目,黑龙江省科研项目
摘    要:从大豆根际分离出的1500多株细菌中筛选出6株生防细菌,其在离体的条件下对大豆根腐病病原菌尖孢镰刀菌(Fusarium oxysporum)有很好的抑制作用,其中菌株18BRR2-2、18BRR9-1和3BRR16-2在离体的条件下也对茄腐镰刀菌(F.solani)、半裸镰刀菌(F.semitectum)和立枯丝核菌(Rhizoctonia solani)有抑制作用,并且这6株生防细菌的发酵液可以抑制尖孢镰刀菌(F.oxysporum)的生长.通过温室盆栽和大田试验表明,这些生防菌对大豆根腐病有很好的防治效果,其中菌株18BRR2-2盆栽和大田的防效均最高,分别达到了45.9%和65.00%.而且这些生防菌也表现出对大豆明显的促生作用.认为18BRR2-2号菌株有开发为防治大豆根腐病生防制剂的潜力.

关 键 词:大豆根腐病  生防细菌  拮抗  防效
文章编号:1000-9841(2005)02-0121-05

EVALUATION OF SIX POTENTIAL BIOCONTROL AGENTS AGAINST SOYBEAN ROOT ROT
Wen Guangyue,Xu Yanli,Li Chunjie,Chen Dong.EVALUATION OF SIX POTENTIAL BIOCONTROL AGENTS AGAINST SOYBEAN ROOT ROT[J].Soybean Science,2005,24(2):121-125.
Authors:Wen Guangyue  Xu Yanli  Li Chunjie  Chen Dong
Abstract:Six strains were screened from 1500 strains, which were isolated from the soybean rhizosphere soil.In vitro, they exhibited the antagonism toward the plant pathogenic Fusarium oxysporum. The strains 18BRR2-2,18BRR9-1,3BRR16-2 among the isolates had the antagonism activity toward F. solani,F. semitectum and Rhizoctonia solani. And, the secondary-metabolite produced by these isolates had the inhibitional activity to F. oxysporum.These isolates significantly reduced the incidence of soybean root rot under controlled glasshouse conditions and the field from 31.97% to 45.90% and 20.00% to 65.00% respectively.And these strains may promote the growth of soybeans seedling. The strain, 18BRR2-2 has the potential to form production to control the soybean root rot.
Keywords:Soybean root rot  Biocontrol bacteria  Antagonism
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