| 固态发酵高温豆粕制备多肽饲料的最优发酵工艺条件研究 |
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| 引用本文： | 刘晓艳,于纯淼,国立东,杨国力,杨庆余.固态发酵高温豆粕制备多肽饲料的最优发酵工艺条件研究[J].大豆科学,2011,30(2):285-289. |
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| 作者姓名： | 刘晓艳 于纯淼 国立东 杨国力 杨庆余 |
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| 作者单位： | 黑龙江中医药大学药学院;齐齐哈尔大学食品与生物工程学院; |
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| 基金项目： | 黑龙江省科技攻关资助项目(GB08B401-03) |
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| 摘 要： | 以高温豆粕为原料,研究了枯草芽孢杆菌固态发酵法制备多肽饲料的最优发酵工艺.首先对影响固态发酵多肽得率的培养基组成进行了研究,采用正交试验确定了最佳的固态发酵培养基为:豆麸比2∶1,加水量110%,加蜜量4%.然后对影响固态发酵中多肽得率的发酵工艺参数进行了研究,采用响应面分析法(RSA)确定最佳发酵工艺条件为:接种量1...
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| 关 键 词： | 高温豆粕 多肽 枯草芽孢杆菌 固态发酵 |
Optimization on Solid State Fermentation Conditions of Producing Polypeptide Feed from High-temperature Soybean Meal |
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| LIU Xiao-yan,YU Chun-miao,GUO Li-dong,YANG Guo-li,YANG Qing-yu.Optimization on Solid State Fermentation Conditions of Producing Polypeptide Feed from High-temperature Soybean Meal[J].Soybean Science,2011,30(2):285-289. |
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| Authors: | LIU Xiao-yan YU Chun-miao GUO Li-dong YANG Guo-li YANG Qing-yu |
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| Institution: | LIU Xiao-yan1,YU Chun-miao1,GUO Li-dong1,YANG Guo-li2,YANG Qing-yu2(1.College of Pharmacy,Heilongjiang University of Traditional Chinese Medicine,Harbin 150030,2.Key Laboratory of Processing Agricultural Products of Heilongjiang Province,College of Food Science and Engineering,Qiqihaer University,Qiqihar 161006,Heilongjiang,China) |
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| Abstract: | The optimal solid state fermentation conditions of polypeptide production feed with Bacillus subtilis from high-temperature soybean meal were studied in this paper.The effects of medium composition on the yield of polypeptide were studied respectively.The orthogonal experiment was designed to obtain optimal fermentation medium,which were soybean meal∶wheat meal 2∶1,adding 110% water and 4% molasses.The effects of fermentation factors on the yield of polypeptide were studied,respectively.The optimal solid state fermentation conditions obtained from response surface methodology were inoculated with 15% of Bacillus subtilis and fermented at 34℃for 72 h,the production rate of soybean polypeptides could reach 46.29%. |
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| Keywords: | High-temperature soybean meal Polypeptide Bacillus subtilis Solid-state fermentation |
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